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Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA
Noroviruses, belonging to the family Caliciviridae, have been identified in human beings and in several animal species including cattle. The distribution of bovine norovirus infections was investigated by both RT-PCR to detect norovirus genomes and a virus-like particles-based ELISA to detect genoty...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117358/ https://www.ncbi.nlm.nih.gov/pubmed/19232845 http://dx.doi.org/10.1016/j.vetmic.2009.01.031 |
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author | Mauroy, Axel Scipioni, Alexandra Mathijs, Elisabeth Saegerman, Claude Mast, Jan Bridger, Janice C. Ziant, Dominique Thys, Christine Thiry, Etienne |
author_facet | Mauroy, Axel Scipioni, Alexandra Mathijs, Elisabeth Saegerman, Claude Mast, Jan Bridger, Janice C. Ziant, Dominique Thys, Christine Thiry, Etienne |
author_sort | Mauroy, Axel |
collection | PubMed |
description | Noroviruses, belonging to the family Caliciviridae, have been identified in human beings and in several animal species including cattle. The distribution of bovine norovirus infections was investigated by both RT-PCR to detect norovirus genomes and a virus-like particles-based ELISA to detect genotype 2 bovine norovirus antibodies. During a 1-year systematic study, a virus prevalence of 7.5% (CI 95%: [3.7; 13.4%]) (10 out of 133 samples) was found in stool samples from diarrhoeic calves screened by RT-PCR. Nucleotide sequencing performed on the polymerase region classified all the norovirus amplicons in the bovine norovirus genotype 2. Rather surprisingly, some rotavirus sequences were also detected. On the basis of the polymerase region, genotype 1 bovine norovirus was not identified. Other enteropathogens were found in all samples. By ELISA, a genotype 2 seroprevalence of 93.2% (CI 95%: [90.4; 95.3%]) was found from calves and adult cattle. Antibody levels against genotype 2 bovine noroviruses rose in the first 6 months of life and were maintained in adults. Together the results of virus prevalence and seroprevalence studies suggest that bovine norovirus infection occurs early in life and that re-infection with serologically related bovine noroviruses strains could occur in adult cattle as reported for rotaviruses. The antibody rise against genotype 2 bovine noroviruses in the adult cattle also suggests a short lived and/or strain specific immunity as already shown in human noroviruses. Genotype 2 bovine noroviruses are endemic in the region investigated. |
format | Online Article Text |
id | pubmed-7117358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71173582020-04-02 Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA Mauroy, Axel Scipioni, Alexandra Mathijs, Elisabeth Saegerman, Claude Mast, Jan Bridger, Janice C. Ziant, Dominique Thys, Christine Thiry, Etienne Vet Microbiol Article Noroviruses, belonging to the family Caliciviridae, have been identified in human beings and in several animal species including cattle. The distribution of bovine norovirus infections was investigated by both RT-PCR to detect norovirus genomes and a virus-like particles-based ELISA to detect genotype 2 bovine norovirus antibodies. During a 1-year systematic study, a virus prevalence of 7.5% (CI 95%: [3.7; 13.4%]) (10 out of 133 samples) was found in stool samples from diarrhoeic calves screened by RT-PCR. Nucleotide sequencing performed on the polymerase region classified all the norovirus amplicons in the bovine norovirus genotype 2. Rather surprisingly, some rotavirus sequences were also detected. On the basis of the polymerase region, genotype 1 bovine norovirus was not identified. Other enteropathogens were found in all samples. By ELISA, a genotype 2 seroprevalence of 93.2% (CI 95%: [90.4; 95.3%]) was found from calves and adult cattle. Antibody levels against genotype 2 bovine noroviruses rose in the first 6 months of life and were maintained in adults. Together the results of virus prevalence and seroprevalence studies suggest that bovine norovirus infection occurs early in life and that re-infection with serologically related bovine noroviruses strains could occur in adult cattle as reported for rotaviruses. The antibody rise against genotype 2 bovine noroviruses in the adult cattle also suggests a short lived and/or strain specific immunity as already shown in human noroviruses. Genotype 2 bovine noroviruses are endemic in the region investigated. Elsevier B.V. 2009-06-12 2009-01-30 /pmc/articles/PMC7117358/ /pubmed/19232845 http://dx.doi.org/10.1016/j.vetmic.2009.01.031 Text en Copyright © 2009 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mauroy, Axel Scipioni, Alexandra Mathijs, Elisabeth Saegerman, Claude Mast, Jan Bridger, Janice C. Ziant, Dominique Thys, Christine Thiry, Etienne Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title | Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title_full | Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title_fullStr | Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title_full_unstemmed | Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title_short | Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA |
title_sort | epidemiological study of bovine norovirus infection by rt-pcr and a vlp-based antibody elisa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117358/ https://www.ncbi.nlm.nih.gov/pubmed/19232845 http://dx.doi.org/10.1016/j.vetmic.2009.01.031 |
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