Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)

A simple and rapid procedure has been developed for the detection of bovine coronaviruses in faecal specimens. The method consists of adsorption of the virus onto mouse erythrocytes at 4°C, removal of unadsorbed material and elution of adsorbed viral material at 37°C. The eluate is then used in a ha...

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Detalles Bibliográficos
Autores principales: Van Balken, J.A.M., De Leeuw, P.W., Ellens, D.J., Straver, P.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1979
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117374/
http://dx.doi.org/10.1016/0378-1135(79)90037-3
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author Van Balken, J.A.M.
De Leeuw, P.W.
Ellens, D.J.
Straver, P.J.
author_facet Van Balken, J.A.M.
De Leeuw, P.W.
Ellens, D.J.
Straver, P.J.
author_sort Van Balken, J.A.M.
collection PubMed
description A simple and rapid procedure has been developed for the detection of bovine coronaviruses in faecal specimens. The method consists of adsorption of the virus onto mouse erythrocytes at 4°C, removal of unadsorbed material and elution of adsorbed viral material at 37°C. The eluate is then used in a haemagglutination test. Specificity of the reaction is checked by a blocking assay. No non-specific reactions have been observed. The sensitivity of the test appeared to be better than that of the electron microscope, at least when crude faecal extracts are used. By ultracentrifugation of the eluates the sensitivity of the assay can be further improved.
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spelling pubmed-71173742020-04-02 Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA) Van Balken, J.A.M. De Leeuw, P.W. Ellens, D.J. Straver, P.J. Vet Microbiol Article A simple and rapid procedure has been developed for the detection of bovine coronaviruses in faecal specimens. The method consists of adsorption of the virus onto mouse erythrocytes at 4°C, removal of unadsorbed material and elution of adsorbed viral material at 37°C. The eluate is then used in a haemagglutination test. Specificity of the reaction is checked by a blocking assay. No non-specific reactions have been observed. The sensitivity of the test appeared to be better than that of the electron microscope, at least when crude faecal extracts are used. By ultracentrifugation of the eluates the sensitivity of the assay can be further improved. Published by Elsevier B.V. 1979-03 2002-11-13 /pmc/articles/PMC7117374/ http://dx.doi.org/10.1016/0378-1135(79)90037-3 Text en Copyright © 1979 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Van Balken, J.A.M.
De Leeuw, P.W.
Ellens, D.J.
Straver, P.J.
Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title_full Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title_fullStr Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title_full_unstemmed Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title_short Detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (HEHA)
title_sort detection of coronavirus in calf faeces with a haemadsorption-elution-haemagglutination assay (heha)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117374/
http://dx.doi.org/10.1016/0378-1135(79)90037-3
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