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Novel genotype of infectious bronchitis virus isolated in China

Recombination events are known to contribute to the emergence of novel infectious bronchitis virus (IBV) genotypes. In this study, we carried out detailed phylogenetic analysis and sequence comparisons based on 74 complete nucleotide sequences of the IBV S1 gene, including strain I0636/16 and 73 rep...

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Detalles Bibliográficos
Autores principales: Ma, Tianxin, Xu, Liwen, Ren, Mengting, Shen, Jie, Han, Zongxi, Sun, Junfeng, Zhao, Yan, Liu, Shengwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117389/
https://www.ncbi.nlm.nih.gov/pubmed/30827386
http://dx.doi.org/10.1016/j.vetmic.2019.01.020
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author Ma, Tianxin
Xu, Liwen
Ren, Mengting
Shen, Jie
Han, Zongxi
Sun, Junfeng
Zhao, Yan
Liu, Shengwang
author_facet Ma, Tianxin
Xu, Liwen
Ren, Mengting
Shen, Jie
Han, Zongxi
Sun, Junfeng
Zhao, Yan
Liu, Shengwang
author_sort Ma, Tianxin
collection PubMed
description Recombination events are known to contribute to the emergence of novel infectious bronchitis virus (IBV) genotypes. In this study, we carried out detailed phylogenetic analysis and sequence comparisons based on 74 complete nucleotide sequences of the IBV S1 gene, including strain I0636/16 and 73 representative sequences from each genotype and lineage. The results showed that strain I0636/16 represented a novel genotype, designated as lineage 1 within genotype VII (GVII-1). Further comparative genomic analysis revealed at least two recombination sites that replaced the spike gene in a lineage 18 within genotype I (GI-18)-like virus with an as-yet-unidentified sequence, likely derived from another IBV strain, resulting a novel serotype with a lower affinity to the respiratory tract in chickens. To the best of our knowledge, this provides the first evidence for recombination leading to replacement of the complete spike gene and the emergence of a novel genotype/serotype with a lower affinity to the respiratory tract in chickens comparing to one of its parental virus ck/CH/LGX/111119. These results emphasize the importance of limiting exposure to novel IBVs that may serve as a source of genetic material for emerging viruses, as well as the importance of IBV surveillance in chicken flocks.
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spelling pubmed-71173892020-04-02 Novel genotype of infectious bronchitis virus isolated in China Ma, Tianxin Xu, Liwen Ren, Mengting Shen, Jie Han, Zongxi Sun, Junfeng Zhao, Yan Liu, Shengwang Vet Microbiol Article Recombination events are known to contribute to the emergence of novel infectious bronchitis virus (IBV) genotypes. In this study, we carried out detailed phylogenetic analysis and sequence comparisons based on 74 complete nucleotide sequences of the IBV S1 gene, including strain I0636/16 and 73 representative sequences from each genotype and lineage. The results showed that strain I0636/16 represented a novel genotype, designated as lineage 1 within genotype VII (GVII-1). Further comparative genomic analysis revealed at least two recombination sites that replaced the spike gene in a lineage 18 within genotype I (GI-18)-like virus with an as-yet-unidentified sequence, likely derived from another IBV strain, resulting a novel serotype with a lower affinity to the respiratory tract in chickens. To the best of our knowledge, this provides the first evidence for recombination leading to replacement of the complete spike gene and the emergence of a novel genotype/serotype with a lower affinity to the respiratory tract in chickens comparing to one of its parental virus ck/CH/LGX/111119. These results emphasize the importance of limiting exposure to novel IBVs that may serve as a source of genetic material for emerging viruses, as well as the importance of IBV surveillance in chicken flocks. Elsevier B.V. 2019-03 2019-01-29 /pmc/articles/PMC7117389/ /pubmed/30827386 http://dx.doi.org/10.1016/j.vetmic.2019.01.020 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ma, Tianxin
Xu, Liwen
Ren, Mengting
Shen, Jie
Han, Zongxi
Sun, Junfeng
Zhao, Yan
Liu, Shengwang
Novel genotype of infectious bronchitis virus isolated in China
title Novel genotype of infectious bronchitis virus isolated in China
title_full Novel genotype of infectious bronchitis virus isolated in China
title_fullStr Novel genotype of infectious bronchitis virus isolated in China
title_full_unstemmed Novel genotype of infectious bronchitis virus isolated in China
title_short Novel genotype of infectious bronchitis virus isolated in China
title_sort novel genotype of infectious bronchitis virus isolated in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117389/
https://www.ncbi.nlm.nih.gov/pubmed/30827386
http://dx.doi.org/10.1016/j.vetmic.2019.01.020
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