The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands

Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rot...

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Autores principales: Rimmelzwaan, G.F., Groen, J., Egberink, H., Borst, G.H.A., UytdeHaag, F.G.C.M., Osterhaus, A.D.M.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117401/
https://www.ncbi.nlm.nih.gov/pubmed/1850889
http://dx.doi.org/10.1016/0378-1135(91)90039-I
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author Rimmelzwaan, G.F.
Groen, J.
Egberink, H.
Borst, G.H.A.
UytdeHaag, F.G.C.M.
Osterhaus, A.D.M.E.
author_facet Rimmelzwaan, G.F.
Groen, J.
Egberink, H.
Borst, G.H.A.
UytdeHaag, F.G.C.M.
Osterhaus, A.D.M.E.
author_sort Rimmelzwaan, G.F.
collection PubMed
description Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands.
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spelling pubmed-71174012020-04-02 The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands Rimmelzwaan, G.F. Groen, J. Egberink, H. Borst, G.H.A. UytdeHaag, F.G.C.M. Osterhaus, A.D.M.E. Vet Microbiol Article Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ELISAs were used to screen samples from dogs in The Netherlands, with or without a history of acute diarrhea. It was shown that the results of the respective serological ELISAs correlated well and that CPV was the major cause of virus-induced acute diarrhea in dogs in The Netherlands. Published by Elsevier B.V. 1991-01 2002-12-10 /pmc/articles/PMC7117401/ /pubmed/1850889 http://dx.doi.org/10.1016/0378-1135(91)90039-I Text en Copyright © 1991 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Rimmelzwaan, G.F.
Groen, J.
Egberink, H.
Borst, G.H.A.
UytdeHaag, F.G.C.M.
Osterhaus, A.D.M.E.
The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title_full The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title_fullStr The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title_full_unstemmed The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title_short The use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in The Netherlands
title_sort use of enzyme-linked immunosorbent assay systems for serology and antigen detection in parvovirus, coronavirus and rotavirus infections in dogs in the netherlands
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117401/
https://www.ncbi.nlm.nih.gov/pubmed/1850889
http://dx.doi.org/10.1016/0378-1135(91)90039-I
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