Cargando…

Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model

To determine differences in infection kinetics of two temporally and genetically different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 2b infection, 62 pigs were randomly assigned to one of seven...

Descripción completa

Detalles Bibliográficos
Autores principales: Opriessnig, Tanja, Gauger, Phillip C., Faaberg, Kay S., Shen, Huigang, Beach, Nathan M., Meng, Xiang-Jin, Wang, Chong, Halbur, Patrick G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117418/
https://www.ncbi.nlm.nih.gov/pubmed/22406346
http://dx.doi.org/10.1016/j.vetmic.2012.02.010
_version_ 1783514366433296384
author Opriessnig, Tanja
Gauger, Phillip C.
Faaberg, Kay S.
Shen, Huigang
Beach, Nathan M.
Meng, Xiang-Jin
Wang, Chong
Halbur, Patrick G.
author_facet Opriessnig, Tanja
Gauger, Phillip C.
Faaberg, Kay S.
Shen, Huigang
Beach, Nathan M.
Meng, Xiang-Jin
Wang, Chong
Halbur, Patrick G.
author_sort Opriessnig, Tanja
collection PubMed
description To determine differences in infection kinetics of two temporally and genetically different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 2b infection, 62 pigs were randomly assigned to one of seven groups: negative controls (n = 8); pigs coinfected with a 1992 PRRSV strain (VR-2385) and PCV2a (CoI-92-2a; n = 9), pigs coinfected with VR-2385 and PCV2b (CoI-92-2b; n = 9), pigs coinfected with a 2006 PRRSV strain (NC16845b) and PCV2a (CoI-06-2a; n = 9), pigs coinfected with NC16845b and PCV2b (CoI-06-2b; n = 9), pigs infected with VR-2385 (n = 9), and pigs infected with NC16845b (n = 9). Blood samples were collected before inoculation and at day post-inoculation (dpi) 3, 6, 9 and 12 and tested for the presence of PRRSV antibody and RNA, PCV2 antibody and DNA, complete blood counts, and interferon gamma (IFN-γ) levels. Regardless of concurrent PCV2 infection, VR-2385 initially replicated at higher levels and reached peak replication levels at dpi 6. Pigs infected with VR-2385 had significantly higher amounts of viral RNA in serum on both dpi 3 and dpi 6, compared to pigs infected with NC16845b. The peak of NC16845b virus replication occurred between dpi 9 and dpi 12 and was associated with a delayed anti-PRRSV antibody response in these pigs. PCV2 coinfection resulted in significantly more severe macroscopic and microscopic lung lesions and a stronger anti-PRRSV IgG response compared to pigs infected with PRRSV alone. This work further emphasizes in vivo replication differences among PRRSV strains and the importance of coinfecting pathogens.
format Online
Article
Text
id pubmed-7117418
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71174182020-04-02 Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model Opriessnig, Tanja Gauger, Phillip C. Faaberg, Kay S. Shen, Huigang Beach, Nathan M. Meng, Xiang-Jin Wang, Chong Halbur, Patrick G. Vet Microbiol Article To determine differences in infection kinetics of two temporally and genetically different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 2b infection, 62 pigs were randomly assigned to one of seven groups: negative controls (n = 8); pigs coinfected with a 1992 PRRSV strain (VR-2385) and PCV2a (CoI-92-2a; n = 9), pigs coinfected with VR-2385 and PCV2b (CoI-92-2b; n = 9), pigs coinfected with a 2006 PRRSV strain (NC16845b) and PCV2a (CoI-06-2a; n = 9), pigs coinfected with NC16845b and PCV2b (CoI-06-2b; n = 9), pigs infected with VR-2385 (n = 9), and pigs infected with NC16845b (n = 9). Blood samples were collected before inoculation and at day post-inoculation (dpi) 3, 6, 9 and 12 and tested for the presence of PRRSV antibody and RNA, PCV2 antibody and DNA, complete blood counts, and interferon gamma (IFN-γ) levels. Regardless of concurrent PCV2 infection, VR-2385 initially replicated at higher levels and reached peak replication levels at dpi 6. Pigs infected with VR-2385 had significantly higher amounts of viral RNA in serum on both dpi 3 and dpi 6, compared to pigs infected with NC16845b. The peak of NC16845b virus replication occurred between dpi 9 and dpi 12 and was associated with a delayed anti-PRRSV antibody response in these pigs. PCV2 coinfection resulted in significantly more severe macroscopic and microscopic lung lesions and a stronger anti-PRRSV IgG response compared to pigs infected with PRRSV alone. This work further emphasizes in vivo replication differences among PRRSV strains and the importance of coinfecting pathogens. Elsevier B.V. 2012-07-06 2012-02-17 /pmc/articles/PMC7117418/ /pubmed/22406346 http://dx.doi.org/10.1016/j.vetmic.2012.02.010 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Opriessnig, Tanja
Gauger, Phillip C.
Faaberg, Kay S.
Shen, Huigang
Beach, Nathan M.
Meng, Xiang-Jin
Wang, Chong
Halbur, Patrick G.
Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title_full Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title_fullStr Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title_full_unstemmed Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title_short Effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
title_sort effect of porcine circovirus type 2a or 2b on infection kinetics and pathogenicity of two genetically divergent strains of porcine reproductive and respiratory syndrome virus in the conventional pig model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117418/
https://www.ncbi.nlm.nih.gov/pubmed/22406346
http://dx.doi.org/10.1016/j.vetmic.2012.02.010
work_keys_str_mv AT opriessnigtanja effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT gaugerphillipc effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT faabergkays effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT shenhuigang effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT beachnathanm effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT mengxiangjin effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT wangchong effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel
AT halburpatrickg effectofporcinecircovirustype2aor2boninfectionkineticsandpathogenicityoftwogeneticallydivergentstrainsofporcinereproductiveandrespiratorysyndromevirusintheconventionalpigmodel