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Purification of infectious bronchitis coronavirus by Sephacryl S-1000 gel chromatography
A procedure was developed to purify infectious bronchitis virus (IBV) by gel chromatography (GC) with a Sephacryl S-1000 column. Virus samples concentrated by centrifugation were applied to a Sephacryl S-1000 column and eluted by 0.02 M phosphate buffer (pH 7.2) containing 0.15 M NaCl. Virus particl...
Autores principales: | Nagano, H., Yagyu, K., Ohta, S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1989
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117437/ https://www.ncbi.nlm.nih.gov/pubmed/2558440 http://dx.doi.org/10.1016/0378-1135(89)90023-0 |
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