Cargando…
Coronavirus infection of spotted hyenas in the Serengeti ecosystem
Sera from 38 free-ranging spotted hyenas (Crocuta crocuta) in the Serengeti ecosystem, Tanzania, were screened for exposure to coronavirus of antigenic group 1. An immunofluorescence assay indicated high levels of exposure to coronavirus among Serengeti hyenas: 95% when considering sera with titer l...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2004
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117438/ https://www.ncbi.nlm.nih.gov/pubmed/15288921 http://dx.doi.org/10.1016/j.vetmic.2004.04.012 |
_version_ | 1783514371023962112 |
---|---|
author | East, Marion L Moestl, Karin Benetka, Viviane Pitra, Christian Höner, Oliver P Wachter, Bettina Hofer, Heribert |
author_facet | East, Marion L Moestl, Karin Benetka, Viviane Pitra, Christian Höner, Oliver P Wachter, Bettina Hofer, Heribert |
author_sort | East, Marion L |
collection | PubMed |
description | Sera from 38 free-ranging spotted hyenas (Crocuta crocuta) in the Serengeti ecosystem, Tanzania, were screened for exposure to coronavirus of antigenic group 1. An immunofluorescence assay indicated high levels of exposure to coronavirus among Serengeti hyenas: 95% when considering sera with titer levels of ≥1:10 and 74% when considering sera with titer levels of ≥1:40. Cubs had generally lower mean titer levels than adults. Exposure among Serengeti hyenas to coronavirus was also confirmed by a serum neutralisation assay and an ELISA. Application of RT-PCR to 27 fecal samples revealed viral RNA in three samples (11%). All three positive fecal samples were from the 15 juvenile animals (<24 months of age) sampled, and none from the 12 adults sampled. No viral RNA was detected in tissue samples (lymph node, intestine, lung) from 11 individuals. Sequencing of two amplified products from the S protein gene of a positive sample revealed the presence of coronavirus specific RNA with a sequence homology to canine coronavirus of 76 and 78% and to feline coronavirus type II of 80 and 84%, respectively. Estimation of the phylogenetic relationship among coronavirus isolates indicated considerable divergence of the hyena variant from those in European, American and Japanese domestic cats and dogs. From long-term observations of several hundred known individuals, the only clinical sign in hyenas consistent with those described for coronavirus infections in dogs and cats was diarrhea. There was no evidence that coronavirus infection in hyenas caused clinical signs similar to feline infectious peritonitis in domestic cats or was a direct cause of mortality in hyenas. To our knowledge, this is the first report of coronavirus infection in Hyaenidae. |
format | Online Article Text |
id | pubmed-7117438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71174382020-04-02 Coronavirus infection of spotted hyenas in the Serengeti ecosystem East, Marion L Moestl, Karin Benetka, Viviane Pitra, Christian Höner, Oliver P Wachter, Bettina Hofer, Heribert Vet Microbiol Article Sera from 38 free-ranging spotted hyenas (Crocuta crocuta) in the Serengeti ecosystem, Tanzania, were screened for exposure to coronavirus of antigenic group 1. An immunofluorescence assay indicated high levels of exposure to coronavirus among Serengeti hyenas: 95% when considering sera with titer levels of ≥1:10 and 74% when considering sera with titer levels of ≥1:40. Cubs had generally lower mean titer levels than adults. Exposure among Serengeti hyenas to coronavirus was also confirmed by a serum neutralisation assay and an ELISA. Application of RT-PCR to 27 fecal samples revealed viral RNA in three samples (11%). All three positive fecal samples were from the 15 juvenile animals (<24 months of age) sampled, and none from the 12 adults sampled. No viral RNA was detected in tissue samples (lymph node, intestine, lung) from 11 individuals. Sequencing of two amplified products from the S protein gene of a positive sample revealed the presence of coronavirus specific RNA with a sequence homology to canine coronavirus of 76 and 78% and to feline coronavirus type II of 80 and 84%, respectively. Estimation of the phylogenetic relationship among coronavirus isolates indicated considerable divergence of the hyena variant from those in European, American and Japanese domestic cats and dogs. From long-term observations of several hundred known individuals, the only clinical sign in hyenas consistent with those described for coronavirus infections in dogs and cats was diarrhea. There was no evidence that coronavirus infection in hyenas caused clinical signs similar to feline infectious peritonitis in domestic cats or was a direct cause of mortality in hyenas. To our knowledge, this is the first report of coronavirus infection in Hyaenidae. Elsevier B.V. 2004-08-19 2004-07-09 /pmc/articles/PMC7117438/ /pubmed/15288921 http://dx.doi.org/10.1016/j.vetmic.2004.04.012 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article East, Marion L Moestl, Karin Benetka, Viviane Pitra, Christian Höner, Oliver P Wachter, Bettina Hofer, Heribert Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title | Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title_full | Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title_fullStr | Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title_full_unstemmed | Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title_short | Coronavirus infection of spotted hyenas in the Serengeti ecosystem |
title_sort | coronavirus infection of spotted hyenas in the serengeti ecosystem |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117438/ https://www.ncbi.nlm.nih.gov/pubmed/15288921 http://dx.doi.org/10.1016/j.vetmic.2004.04.012 |
work_keys_str_mv | AT eastmarionl coronavirusinfectionofspottedhyenasintheserengetiecosystem AT moestlkarin coronavirusinfectionofspottedhyenasintheserengetiecosystem AT benetkaviviane coronavirusinfectionofspottedhyenasintheserengetiecosystem AT pitrachristian coronavirusinfectionofspottedhyenasintheserengetiecosystem AT honeroliverp coronavirusinfectionofspottedhyenasintheserengetiecosystem AT wachterbettina coronavirusinfectionofspottedhyenasintheserengetiecosystem AT hoferheribert coronavirusinfectionofspottedhyenasintheserengetiecosystem |