Cargando…

Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea

The spike (S) glycoprotein of transmissible gastroenteritis virus (TGEV) is the predominant inducer of neutralizing antibodies and has been implicated in virulence and host cell tropism. In this study, the nucleotide and deduced amino acid sequences of the amino terminal half of the S glycoprotein g...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Soo Joung, Han, Jeong Hee, Kwon, Hyuk Moo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117453/
https://www.ncbi.nlm.nih.gov/pubmed/12814887
http://dx.doi.org/10.1016/S0378-1135(03)00101-9
_version_ 1783514374583877632
author Kim, Soo Joung
Han, Jeong Hee
Kwon, Hyuk Moo
author_facet Kim, Soo Joung
Han, Jeong Hee
Kwon, Hyuk Moo
author_sort Kim, Soo Joung
collection PubMed
description The spike (S) glycoprotein of transmissible gastroenteritis virus (TGEV) is the predominant inducer of neutralizing antibodies and has been implicated in virulence and host cell tropism. In this study, the nucleotide and deduced amino acid sequences of the amino terminal half of the S glycoprotein gene of one Korean field TGEV strain (133) isolated in 1997 and three Korean field TGEV strains (KT2, KT3 and KT4) isolated in 2000 and HKT2 strain, KT2 passaged 104 times in ST cells, were determined. The amino terminal half of the S glycoprotein gene including antigenic sites A, B, C and D, were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). Amplified PCR products were cloned, sequenced, and compared with published sequences for non-Korean TGEV strains. Korea TGEV field strains had 98.5–99.5% nucleotide sequence and 97.2–99.0% amino acid sequence similarity with each other. They had 96.5–99.0% nucleotide sequence similarity and 94.9–97.6% amino acid sequence similarity compared to non-Korean TGEV strains. Korean TGEV strains had several specific nucleotide and amino acid sequences which were not found in foreign TGEV or PRCV strains. HKT2 strain differed by 0.89% in nucleotide and 2.03% amino acid sequences compared to original KT2 strain although the regions forming four antigenic sites were not changed. By phylogenetic tree analysis, Korean field TGEV strains were branched into different groups from non-Korean TGEV or PRCV strains. Korean TGEV field strains KT2 and 133 were branched in separate groups that were differentiated from the other Korean TGEV strains. The Korean TGEV strains seemed to be evolved from a separate lineage of TGEV strain.
format Online
Article
Text
id pubmed-7117453
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher Elsevier Science B.V.
record_format MEDLINE/PubMed
spelling pubmed-71174532020-04-02 Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea Kim, Soo Joung Han, Jeong Hee Kwon, Hyuk Moo Vet Microbiol Article The spike (S) glycoprotein of transmissible gastroenteritis virus (TGEV) is the predominant inducer of neutralizing antibodies and has been implicated in virulence and host cell tropism. In this study, the nucleotide and deduced amino acid sequences of the amino terminal half of the S glycoprotein gene of one Korean field TGEV strain (133) isolated in 1997 and three Korean field TGEV strains (KT2, KT3 and KT4) isolated in 2000 and HKT2 strain, KT2 passaged 104 times in ST cells, were determined. The amino terminal half of the S glycoprotein gene including antigenic sites A, B, C and D, were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). Amplified PCR products were cloned, sequenced, and compared with published sequences for non-Korean TGEV strains. Korea TGEV field strains had 98.5–99.5% nucleotide sequence and 97.2–99.0% amino acid sequence similarity with each other. They had 96.5–99.0% nucleotide sequence similarity and 94.9–97.6% amino acid sequence similarity compared to non-Korean TGEV strains. Korean TGEV strains had several specific nucleotide and amino acid sequences which were not found in foreign TGEV or PRCV strains. HKT2 strain differed by 0.89% in nucleotide and 2.03% amino acid sequences compared to original KT2 strain although the regions forming four antigenic sites were not changed. By phylogenetic tree analysis, Korean field TGEV strains were branched into different groups from non-Korean TGEV or PRCV strains. Korean TGEV field strains KT2 and 133 were branched in separate groups that were differentiated from the other Korean TGEV strains. The Korean TGEV strains seemed to be evolved from a separate lineage of TGEV strain. Elsevier Science B.V. 2003-07-17 2003-05-27 /pmc/articles/PMC7117453/ /pubmed/12814887 http://dx.doi.org/10.1016/S0378-1135(03)00101-9 Text en Copyright © 2003 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kim, Soo Joung
Han, Jeong Hee
Kwon, Hyuk Moo
Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title_full Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title_fullStr Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title_full_unstemmed Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title_short Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea
title_sort partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in korea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117453/
https://www.ncbi.nlm.nih.gov/pubmed/12814887
http://dx.doi.org/10.1016/S0378-1135(03)00101-9
work_keys_str_mv AT kimsoojoung partialsequenceofthespikeglycoproteingeneoftransmissiblegastroenteritisvirusesisolatedinkorea
AT hanjeonghee partialsequenceofthespikeglycoproteingeneoftransmissiblegastroenteritisvirusesisolatedinkorea
AT kwonhyukmoo partialsequenceofthespikeglycoproteingeneoftransmissiblegastroenteritisvirusesisolatedinkorea