EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication

Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Using the method of GST pull-down with the nucleocapsid (N), N protein was found to interact with swine testes...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xin, Shi, Hongyan, Chen, Jianfei, Shi, Da, Li, Changlong, Feng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117464/
https://www.ncbi.nlm.nih.gov/pubmed/24974120
http://dx.doi.org/10.1016/j.vetmic.2014.05.034
_version_ 1783514377095217152
author Zhang, Xin
Shi, Hongyan
Chen, Jianfei
Shi, Da
Li, Changlong
Feng, Li
author_facet Zhang, Xin
Shi, Hongyan
Chen, Jianfei
Shi, Da
Li, Changlong
Feng, Li
author_sort Zhang, Xin
collection PubMed
description Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Using the method of GST pull-down with the nucleocapsid (N), N protein was found to interact with swine testes (ST) cells elongation factor 1-alpha (EF1A), an essential component of the translational machinery with an important role in cells. In vitro and in virus-infected cells interaction was then confirmed by co-precipitation. Knockdown of EF1A impairs N protein proliferation and TGEV replication in host cell. It was demonstrated that EF1A plays a role in TGEV replication. The present study thus provides a protein-related information that should be useful for underlying mechanism of coronavirus replication.
format Online
Article
Text
id pubmed-7117464
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71174642020-04-02 EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication Zhang, Xin Shi, Hongyan Chen, Jianfei Shi, Da Li, Changlong Feng, Li Vet Microbiol Article Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Using the method of GST pull-down with the nucleocapsid (N), N protein was found to interact with swine testes (ST) cells elongation factor 1-alpha (EF1A), an essential component of the translational machinery with an important role in cells. In vitro and in virus-infected cells interaction was then confirmed by co-precipitation. Knockdown of EF1A impairs N protein proliferation and TGEV replication in host cell. It was demonstrated that EF1A plays a role in TGEV replication. The present study thus provides a protein-related information that should be useful for underlying mechanism of coronavirus replication. Elsevier B.V. 2014-08-27 2014-06-09 /pmc/articles/PMC7117464/ /pubmed/24974120 http://dx.doi.org/10.1016/j.vetmic.2014.05.034 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Xin
Shi, Hongyan
Chen, Jianfei
Shi, Da
Li, Changlong
Feng, Li
EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title_full EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title_fullStr EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title_full_unstemmed EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title_short EF1A interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
title_sort ef1a interacting with nucleocapsid protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117464/
https://www.ncbi.nlm.nih.gov/pubmed/24974120
http://dx.doi.org/10.1016/j.vetmic.2014.05.034
work_keys_str_mv AT zhangxin ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication
AT shihongyan ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication
AT chenjianfei ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication
AT shida ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication
AT lichanglong ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication
AT fengli ef1ainteractingwithnucleocapsidproteinoftransmissiblegastroenteritiscoronavirusandplaysaroleinvirusreplication

Ejemplares similares