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Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis
Feline infectious peritonitis (FIP) is a fatal, coronavirus-induced systemic disease in domestic and wild felids. The pathology associated with FIP (multifocal granulomatous vasculitis) is considered to be elicited by exaggerated activation and subsequent extravasation of leukocytes. As changes in t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117481/ https://www.ncbi.nlm.nih.gov/pubmed/23910523 http://dx.doi.org/10.1016/j.vetmic.2013.06.027 |
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author | Olyslaegers, Dominique A.J. Dedeurwaerder, Annelike Desmarets, Lowiese M.B. Vermeulen, Ben L. Dewerchin, Hannah L. Nauwynck, Hans J. |
author_facet | Olyslaegers, Dominique A.J. Dedeurwaerder, Annelike Desmarets, Lowiese M.B. Vermeulen, Ben L. Dewerchin, Hannah L. Nauwynck, Hans J. |
author_sort | Olyslaegers, Dominique A.J. |
collection | PubMed |
description | Feline infectious peritonitis (FIP) is a fatal, coronavirus-induced systemic disease in domestic and wild felids. The pathology associated with FIP (multifocal granulomatous vasculitis) is considered to be elicited by exaggerated activation and subsequent extravasation of leukocytes. As changes in the expression of adhesion molecules on circulating leukocytes precede their margination and emigration, we reasoned that the expression of leukocyte adhesion molecules may be altered in FIP. In present study, the expression of principal adhesion molecules involved in leukocyte transmigration (CD15s, CD11a, CD11b, CD18, CD49d, and CD54) on peripheral blood leukocytes from cats with naturally occurring FIP (n = 15) and controls (n = 12) was quantified by flow cytometry using a formaldehyde-based rapid leukocyte preparation technique. T- and B-lymphocytes from FIP patients exhibit higher expression of both subunits (CD11a and CD18) composing the β(2) integrin lymphocyte function-associated antigen (LFA)-1. In addition, the expression of the α(4) subunit (CD49d) of the β(1) integrin very late antigen (VLA)-4 was elevated on B-lymphocytes from FIP patients. The expression of CD11b and CD18, that combine to form the β(2) integrin macrophage-1 antigen (Mac-1), was elevated on monocytes, whereas the density of CD49d was reduced on this population in FIP. Granulocytes of FIP cats displayed an increased expression of the α chain of Mac-1 (CD11b). These observations suggest that leukocytes from FIP patients show signs of systemic activation causing them to extravasate into surrounding tissues and ultimately contribute to pyogranuloma formation seen in FIP. |
format | Online Article Text |
id | pubmed-7117481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71174812020-04-02 Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis Olyslaegers, Dominique A.J. Dedeurwaerder, Annelike Desmarets, Lowiese M.B. Vermeulen, Ben L. Dewerchin, Hannah L. Nauwynck, Hans J. Vet Microbiol Article Feline infectious peritonitis (FIP) is a fatal, coronavirus-induced systemic disease in domestic and wild felids. The pathology associated with FIP (multifocal granulomatous vasculitis) is considered to be elicited by exaggerated activation and subsequent extravasation of leukocytes. As changes in the expression of adhesion molecules on circulating leukocytes precede their margination and emigration, we reasoned that the expression of leukocyte adhesion molecules may be altered in FIP. In present study, the expression of principal adhesion molecules involved in leukocyte transmigration (CD15s, CD11a, CD11b, CD18, CD49d, and CD54) on peripheral blood leukocytes from cats with naturally occurring FIP (n = 15) and controls (n = 12) was quantified by flow cytometry using a formaldehyde-based rapid leukocyte preparation technique. T- and B-lymphocytes from FIP patients exhibit higher expression of both subunits (CD11a and CD18) composing the β(2) integrin lymphocyte function-associated antigen (LFA)-1. In addition, the expression of the α(4) subunit (CD49d) of the β(1) integrin very late antigen (VLA)-4 was elevated on B-lymphocytes from FIP patients. The expression of CD11b and CD18, that combine to form the β(2) integrin macrophage-1 antigen (Mac-1), was elevated on monocytes, whereas the density of CD49d was reduced on this population in FIP. Granulocytes of FIP cats displayed an increased expression of the α chain of Mac-1 (CD11b). These observations suggest that leukocytes from FIP patients show signs of systemic activation causing them to extravasate into surrounding tissues and ultimately contribute to pyogranuloma formation seen in FIP. Elsevier B.V. 2013-10-25 2013-07-09 /pmc/articles/PMC7117481/ /pubmed/23910523 http://dx.doi.org/10.1016/j.vetmic.2013.06.027 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Olyslaegers, Dominique A.J. Dedeurwaerder, Annelike Desmarets, Lowiese M.B. Vermeulen, Ben L. Dewerchin, Hannah L. Nauwynck, Hans J. Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title | Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title_full | Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title_fullStr | Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title_full_unstemmed | Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title_short | Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
title_sort | altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117481/ https://www.ncbi.nlm.nih.gov/pubmed/23910523 http://dx.doi.org/10.1016/j.vetmic.2013.06.027 |
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