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Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus

The antigenic structure of the S glycoprotein of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) has been determined and correlated with the physical structure. Four antigenic sites have been defined (A, B, C, and D). The sites involved in the neutralization of...

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Autores principales: Enjuanes, Luis, Suñé, Carlos, Gebauer, Fátima, Smerdou, Cristian, Camacho, Ana, Antón, Inés M., González, Silvia, Talamillo, Ana, Méndez, Ana, Ballesteros, María L., Sánchez, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117487/
https://www.ncbi.nlm.nih.gov/pubmed/1282756
http://dx.doi.org/10.1016/0378-1135(92)90053-V
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author Enjuanes, Luis
Suñé, Carlos
Gebauer, Fátima
Smerdou, Cristian
Camacho, Ana
Antón, Inés M.
González, Silvia
Talamillo, Ana
Méndez, Ana
Ballesteros, María L.
Sánchez, Carlos
author_facet Enjuanes, Luis
Suñé, Carlos
Gebauer, Fátima
Smerdou, Cristian
Camacho, Ana
Antón, Inés M.
González, Silvia
Talamillo, Ana
Méndez, Ana
Ballesteros, María L.
Sánchez, Carlos
author_sort Enjuanes, Luis
collection PubMed
description The antigenic structure of the S glycoprotein of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) has been determined and correlated with the physical structure. Four antigenic sites have been defined (A, B, C, and D). The sites involved in the neutralization of TGEV are: A, D, and B, sites A and D being antigenically dominant for TGEV neutralization in vitro. These two sites have specific properties of interest: site A is highly conserved and is present in coronaviruses of three animal species, and site D can be represented by synthetic peptides. Both sites might be relevant in protection in vivo. PRCV does not have sites B and C, due to a genomic deletion. Complex antigenic sites, i.e., conformation and glycosylation dependent sites, have been represented by simple mimotopes selected from a library expressing recombinant peptides with random sequences, or by anti-idiotypic internal image monoclonal antibodies. An epidemiological tree relating the TGEVs and PRCVs has been proposed. The estimated mutation fixation rate of 7 ± 2 × 10(−4) substitutions per nucleotide and year indicates that TGEV related coronaviruses show similar variability to other RNA viruses. In order to induce secretory immunity, different segments of the S gene have been expressed using avirulent forms of Salmonella typhimurium and adenovirus. These vectors, with a tropism for Peyer's patches may be ideal candidates in protection against TGEV.
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spelling pubmed-71174872020-04-02 Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus Enjuanes, Luis Suñé, Carlos Gebauer, Fátima Smerdou, Cristian Camacho, Ana Antón, Inés M. González, Silvia Talamillo, Ana Méndez, Ana Ballesteros, María L. Sánchez, Carlos Vet Microbiol Article The antigenic structure of the S glycoprotein of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) has been determined and correlated with the physical structure. Four antigenic sites have been defined (A, B, C, and D). The sites involved in the neutralization of TGEV are: A, D, and B, sites A and D being antigenically dominant for TGEV neutralization in vitro. These two sites have specific properties of interest: site A is highly conserved and is present in coronaviruses of three animal species, and site D can be represented by synthetic peptides. Both sites might be relevant in protection in vivo. PRCV does not have sites B and C, due to a genomic deletion. Complex antigenic sites, i.e., conformation and glycosylation dependent sites, have been represented by simple mimotopes selected from a library expressing recombinant peptides with random sequences, or by anti-idiotypic internal image monoclonal antibodies. An epidemiological tree relating the TGEVs and PRCVs has been proposed. The estimated mutation fixation rate of 7 ± 2 × 10(−4) substitutions per nucleotide and year indicates that TGEV related coronaviruses show similar variability to other RNA viruses. In order to induce secretory immunity, different segments of the S gene have been expressed using avirulent forms of Salmonella typhimurium and adenovirus. These vectors, with a tropism for Peyer's patches may be ideal candidates in protection against TGEV. Published by Elsevier B.V. 1992-11 2002-11-13 /pmc/articles/PMC7117487/ /pubmed/1282756 http://dx.doi.org/10.1016/0378-1135(92)90053-V Text en Copyright © 1992 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Enjuanes, Luis
Suñé, Carlos
Gebauer, Fátima
Smerdou, Cristian
Camacho, Ana
Antón, Inés M.
González, Silvia
Talamillo, Ana
Méndez, Ana
Ballesteros, María L.
Sánchez, Carlos
Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title_full Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title_fullStr Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title_full_unstemmed Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title_short Antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
title_sort antigen selection and presentation to protect against transmissible gastroenteritis coronavirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117487/
https://www.ncbi.nlm.nih.gov/pubmed/1282756
http://dx.doi.org/10.1016/0378-1135(92)90053-V
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