Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies

An enzyme-linked immunosorbent assay (ELISA) using a detergent-solubilized antigen of purified virus was developed for detection of antibody against porcine transmissible gastroenteritis (TGE) virus in swine serum. The ELISA demonstrated antibody responses in pigs immunized intramuscularly with the...

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Autores principales: Hohdatsu, T., Eiguchi, Y., Ide, S., Baba, K., Yamagishi, H., Kume, T., Matumoto, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1987
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117489/
https://www.ncbi.nlm.nih.gov/pubmed/3027955
http://dx.doi.org/10.1016/0378-1135(87)90103-9
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author Hohdatsu, T.
Eiguchi, Y.
Ide, S.
Baba, K.
Yamagishi, H.
Kume, T.
Matumoto, M.
author_facet Hohdatsu, T.
Eiguchi, Y.
Ide, S.
Baba, K.
Yamagishi, H.
Kume, T.
Matumoto, M.
author_sort Hohdatsu, T.
collection PubMed
description An enzyme-linked immunosorbent assay (ELISA) using a detergent-solubilized antigen of purified virus was developed for detection of antibody against porcine transmissible gastroenteritis (TGE) virus in swine serum. The ELISA demonstrated antibody responses in pigs immunized intramuscularly with the attenuated TO-163 strain of TGE virus and in pigs orally infected with the virulent Shizuoka strain of the virus. The results of the ELISA were well correlated with those of the neutralization test. These results indicate the usefulness of the ELISA as a serological tool for TGE virus antibody.
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spelling pubmed-71174892020-04-02 Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies Hohdatsu, T. Eiguchi, Y. Ide, S. Baba, K. Yamagishi, H. Kume, T. Matumoto, M. Vet Microbiol Article An enzyme-linked immunosorbent assay (ELISA) using a detergent-solubilized antigen of purified virus was developed for detection of antibody against porcine transmissible gastroenteritis (TGE) virus in swine serum. The ELISA demonstrated antibody responses in pigs immunized intramuscularly with the attenuated TO-163 strain of TGE virus and in pigs orally infected with the virulent Shizuoka strain of the virus. The results of the ELISA were well correlated with those of the neutralization test. These results indicate the usefulness of the ELISA as a serological tool for TGE virus antibody. Published by Elsevier B.V. 1987-01 2002-11-13 /pmc/articles/PMC7117489/ /pubmed/3027955 http://dx.doi.org/10.1016/0378-1135(87)90103-9 Text en Copyright © 1987 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hohdatsu, T.
Eiguchi, Y.
Ide, S.
Baba, K.
Yamagishi, H.
Kume, T.
Matumoto, M.
Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title_full Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title_fullStr Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title_full_unstemmed Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title_short Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
title_sort evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117489/
https://www.ncbi.nlm.nih.gov/pubmed/3027955
http://dx.doi.org/10.1016/0378-1135(87)90103-9
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