The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens

Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) prot...

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Detalles Bibliográficos
Autores principales: Tian, Lang, Wang, Hong-ning, Lu, Dan, Zhang, Yun-fei, Wang, Ting, Kang, Run-ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117539/
https://www.ncbi.nlm.nih.gov/pubmed/18840402
http://dx.doi.org/10.1016/j.bbrc.2008.09.125
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author Tian, Lang
Wang, Hong-ning
Lu, Dan
Zhang, Yun-fei
Wang, Ting
Kang, Run-ming
author_facet Tian, Lang
Wang, Hong-ning
Lu, Dan
Zhang, Yun-fei
Wang, Ting
Kang, Run-ming
author_sort Tian, Lang
collection PubMed
description Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine. The 7-day-old chickens were immunized intramuscularly with multi-epitope DNA vaccine encapsulated by liposome and boosted two weeks later, and were challenged by virulent IBV strain five weeks post booster. The results showed that multi-epitope DNA vaccine led to a dramatic augmentation of humoral and cellular responses, and provided up to 80.0% rate of immune protection. The novel immunogenic chimeric multi-epitope DNA vaccine revealed in this study provided a new candidate target for IBV vaccine development.
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spelling pubmed-71175392020-04-02 The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens Tian, Lang Wang, Hong-ning Lu, Dan Zhang, Yun-fei Wang, Ting Kang, Run-ming Biochem Biophys Res Commun Article Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine. The 7-day-old chickens were immunized intramuscularly with multi-epitope DNA vaccine encapsulated by liposome and boosted two weeks later, and were challenged by virulent IBV strain five weeks post booster. The results showed that multi-epitope DNA vaccine led to a dramatic augmentation of humoral and cellular responses, and provided up to 80.0% rate of immune protection. The novel immunogenic chimeric multi-epitope DNA vaccine revealed in this study provided a new candidate target for IBV vaccine development. Elsevier Inc. 2008-12-05 2008-10-07 /pmc/articles/PMC7117539/ /pubmed/18840402 http://dx.doi.org/10.1016/j.bbrc.2008.09.125 Text en Copyright © 2008 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Tian, Lang
Wang, Hong-ning
Lu, Dan
Zhang, Yun-fei
Wang, Ting
Kang, Run-ming
The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title_full The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title_fullStr The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title_full_unstemmed The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title_short The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
title_sort immunoreactivity of a chimeric multi-epitope dna vaccine against ibv in chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7117539/
https://www.ncbi.nlm.nih.gov/pubmed/18840402
http://dx.doi.org/10.1016/j.bbrc.2008.09.125
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