Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells

CRISPR-based screening methods using single-cell RNA sequencing (scRNA-seq) technology enable comprehensive profiling of gene perturbations from knock-out mutations. However, evaluating substitution mutations using scRNA-seq is currently limited. We combined CRISPR RNA-guided deaminase and scRNA-seq...

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Detalles Bibliográficos
Autores principales: Jun, Soyeong, Lim, Hyeonseob, Chun, Honggu, Lee, Ji Hyun, Bang, Duhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118117/
https://www.ncbi.nlm.nih.gov/pubmed/32242071
http://dx.doi.org/10.1038/s42003-020-0888-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118117/
https://www.ncbi.nlm.nih.gov/pubmed/32242071
http://dx.doi.org/10.1038/s42003-020-0888-2

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