Cargando…
Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India
Pneumonia in bovines is a multifactorial disease manifestation leading to heavy economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPI-3) are among the important contributing factors for the development of pneumonia in young animals. These vira...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118649/ https://www.ncbi.nlm.nih.gov/pubmed/32205208 http://dx.doi.org/10.1016/j.micpath.2020.104165 |
_version_ | 1783514605025230848 |
---|---|
author | Kamdi, Bhupesh Singh, Rajendra Singh, Vidya Singh, Shailendra Kumar, Pawan Singh, Karam Pal George, Neethu Dhama, Kuldeep |
author_facet | Kamdi, Bhupesh Singh, Rajendra Singh, Vidya Singh, Shailendra Kumar, Pawan Singh, Karam Pal George, Neethu Dhama, Kuldeep |
author_sort | Kamdi, Bhupesh |
collection | PubMed |
description | Pneumonia in bovines is a multifactorial disease manifestation leading to heavy economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPI-3) are among the important contributing factors for the development of pneumonia in young animals. These viral agents either primarily cause pneumonia or predispose animals to the development of pneumonia. Although, the role of BRSV and BPI-3 in the pathogenesis of pneumonia is well established, there are no reports of involvement of BRSV and BPI-3 from Indian cattle and buffaloes suffering from pneumonia. In the present investigation, we performed postmortem examinations of 406 cattle and buffaloes, which were below twelve months of age. Out of 406 cases, twelve (2.95%) cases were positive for BRSV and fifteen (3.69%) cases were positive for BPI-3, screened by reverse transcriptase polymerase chain reaction (RT-PCR). Further, positive cases were confirmed by sequence analysis of RT-PCR amplicons and direct immunofluorescence antibody test (d-FAT) in paraffin-embedded lung tissue sections. BRSV positive cases revealed characteristic findings of bronchiolar epithelial necrosis, thickened alveolar septa by mononuclear cells infiltration and edema; alveolar lumens were filled with mononuclear cells and numerous syncytial cells were seen having intracytoplasmic inclusions. The BRSV antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. In fifteen cases, where BPI-3 was detected, bronchointerstitial pneumonia in the majority of cases with thickened alveolar septa by mild macrophage infiltration, hyperplasia of type-II pneumocytes and bronchiolar necrosis along with syncytial cells having intracytoplasmic inclusions in the majority of cases were observed. The BPI-3 antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. RT-PCR amplicons of BRSV and BPI-3 obtained were sequenced and their analysis showed homology with already available sequences in the NCBI database. It is the first report of detection of BRSV and BPI-3 from pneumonic cases by RT-PCR and d-FAT from cattle and buffaloes of India, indicating the need for more epidemiological studies. |
format | Online Article Text |
id | pubmed-7118649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71186492020-04-03 Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India Kamdi, Bhupesh Singh, Rajendra Singh, Vidya Singh, Shailendra Kumar, Pawan Singh, Karam Pal George, Neethu Dhama, Kuldeep Microb Pathog Short Communication Pneumonia in bovines is a multifactorial disease manifestation leading to heavy economic losses. Infections of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPI-3) are among the important contributing factors for the development of pneumonia in young animals. These viral agents either primarily cause pneumonia or predispose animals to the development of pneumonia. Although, the role of BRSV and BPI-3 in the pathogenesis of pneumonia is well established, there are no reports of involvement of BRSV and BPI-3 from Indian cattle and buffaloes suffering from pneumonia. In the present investigation, we performed postmortem examinations of 406 cattle and buffaloes, which were below twelve months of age. Out of 406 cases, twelve (2.95%) cases were positive for BRSV and fifteen (3.69%) cases were positive for BPI-3, screened by reverse transcriptase polymerase chain reaction (RT-PCR). Further, positive cases were confirmed by sequence analysis of RT-PCR amplicons and direct immunofluorescence antibody test (d-FAT) in paraffin-embedded lung tissue sections. BRSV positive cases revealed characteristic findings of bronchiolar epithelial necrosis, thickened alveolar septa by mononuclear cells infiltration and edema; alveolar lumens were filled with mononuclear cells and numerous syncytial cells were seen having intracytoplasmic inclusions. The BRSV antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. In fifteen cases, where BPI-3 was detected, bronchointerstitial pneumonia in the majority of cases with thickened alveolar septa by mild macrophage infiltration, hyperplasia of type-II pneumocytes and bronchiolar necrosis along with syncytial cells having intracytoplasmic inclusions in the majority of cases were observed. The BPI-3 antigen distribution was found to be in bronchiolar and alveolar epithelium and syncytial cells in the lung sections. RT-PCR amplicons of BRSV and BPI-3 obtained were sequenced and their analysis showed homology with already available sequences in the NCBI database. It is the first report of detection of BRSV and BPI-3 from pneumonic cases by RT-PCR and d-FAT from cattle and buffaloes of India, indicating the need for more epidemiological studies. Elsevier Ltd. 2020-08 2020-03-20 /pmc/articles/PMC7118649/ /pubmed/32205208 http://dx.doi.org/10.1016/j.micpath.2020.104165 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Kamdi, Bhupesh Singh, Rajendra Singh, Vidya Singh, Shailendra Kumar, Pawan Singh, Karam Pal George, Neethu Dhama, Kuldeep Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title | Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title_full | Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title_fullStr | Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title_full_unstemmed | Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title_short | Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India |
title_sort | immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from india |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118649/ https://www.ncbi.nlm.nih.gov/pubmed/32205208 http://dx.doi.org/10.1016/j.micpath.2020.104165 |
work_keys_str_mv | AT kamdibhupesh immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT singhrajendra immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT singhvidya immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT singhshailendra immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT kumarpawan immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT singhkarampal immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT georgeneethu immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia AT dhamakuldeep immunofluorescenceandmoleculardiagnosisofbovinerespiratorysyncytialvirusandbovineparainfluenzavirusinthenaturallyinfectedyoungcattleandbuffaloesfromindia |