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Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression

BACKGROUND: Cissus quadrangularis Linn. (CQ) has been used in Indian and Thai traditional medicine for healing bone fractures because of numerous active ingredients in CQ. It is still unclear which compounds are the active ingredients for bone formation. METHODS: The molecular docking technique, the...

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Autores principales: Ruangsuriya, Jetsada, Charumanee, Suporn, Jiranusornkul, Supat, Sirisa-ard, Panee, Sirithunyalug, Busaban, Sirithunyalug, Jakkapan, Pattananandecha, Thanawat, Saenjum, Chalermpong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119164/
https://www.ncbi.nlm.nih.gov/pubmed/32245457
http://dx.doi.org/10.1186/s12906-020-02892-w
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author Ruangsuriya, Jetsada
Charumanee, Suporn
Jiranusornkul, Supat
Sirisa-ard, Panee
Sirithunyalug, Busaban
Sirithunyalug, Jakkapan
Pattananandecha, Thanawat
Saenjum, Chalermpong
author_facet Ruangsuriya, Jetsada
Charumanee, Suporn
Jiranusornkul, Supat
Sirisa-ard, Panee
Sirithunyalug, Busaban
Sirithunyalug, Jakkapan
Pattananandecha, Thanawat
Saenjum, Chalermpong
author_sort Ruangsuriya, Jetsada
collection PubMed
description BACKGROUND: Cissus quadrangularis Linn. (CQ) has been used in Indian and Thai traditional medicine for healing bone fractures because of numerous active ingredients in CQ. It is still unclear which compounds are the active ingredients for bone formation. METHODS: The molecular docking technique, the ethanolic extraction along with hexane fractionation, and an in vitro experiment with a human osteoblast cell line (MG-63) were used to narrow down the active compounds, to prepare the CQ extract, and to test biological activities, respectively. RESULTS: The molecular docking technique revealed that quercetin and β-sitosterol had highest and lowest potential to bind to estrogen receptors, respectively. Compared to the crude ethanol extract (P1), the ethanolic fraction (P2) was enriched with rutin and quercetin at 65.36 ± 0.75 and 1.06 ± 0.12 mg/g, respectively. Alkaline phosphatase (ALP) activity was significantly enhanced in osteoblasts exposed to the P2 in both tested concentrations. The amount of hydroxyproline was slightly increased in the P1 treatment, while osteocalcin was inhibited. Moreover, the P2 significantly activated osteoprotegerin (OPG) and inhibited receptor activator of nuclear factor κ ligand (RANKL) expression. CONCLUSIONS: Taken together, the enriched rutin and quercetin fraction of CQ triggered the molecules involved in bone formation and the molecules inhibiting bone resorption.
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spelling pubmed-71191642020-04-07 Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression Ruangsuriya, Jetsada Charumanee, Suporn Jiranusornkul, Supat Sirisa-ard, Panee Sirithunyalug, Busaban Sirithunyalug, Jakkapan Pattananandecha, Thanawat Saenjum, Chalermpong BMC Complement Med Ther Research Article BACKGROUND: Cissus quadrangularis Linn. (CQ) has been used in Indian and Thai traditional medicine for healing bone fractures because of numerous active ingredients in CQ. It is still unclear which compounds are the active ingredients for bone formation. METHODS: The molecular docking technique, the ethanolic extraction along with hexane fractionation, and an in vitro experiment with a human osteoblast cell line (MG-63) were used to narrow down the active compounds, to prepare the CQ extract, and to test biological activities, respectively. RESULTS: The molecular docking technique revealed that quercetin and β-sitosterol had highest and lowest potential to bind to estrogen receptors, respectively. Compared to the crude ethanol extract (P1), the ethanolic fraction (P2) was enriched with rutin and quercetin at 65.36 ± 0.75 and 1.06 ± 0.12 mg/g, respectively. Alkaline phosphatase (ALP) activity was significantly enhanced in osteoblasts exposed to the P2 in both tested concentrations. The amount of hydroxyproline was slightly increased in the P1 treatment, while osteocalcin was inhibited. Moreover, the P2 significantly activated osteoprotegerin (OPG) and inhibited receptor activator of nuclear factor κ ligand (RANKL) expression. CONCLUSIONS: Taken together, the enriched rutin and quercetin fraction of CQ triggered the molecules involved in bone formation and the molecules inhibiting bone resorption. BioMed Central 2020-04-03 /pmc/articles/PMC7119164/ /pubmed/32245457 http://dx.doi.org/10.1186/s12906-020-02892-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Ruangsuriya, Jetsada
Charumanee, Suporn
Jiranusornkul, Supat
Sirisa-ard, Panee
Sirithunyalug, Busaban
Sirithunyalug, Jakkapan
Pattananandecha, Thanawat
Saenjum, Chalermpong
Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title_full Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title_fullStr Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title_full_unstemmed Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title_short Depletion of β-sitosterol and enrichment of quercetin and rutin in Cissus quadrangularis Linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
title_sort depletion of β-sitosterol and enrichment of quercetin and rutin in cissus quadrangularis linn fraction enhanced osteogenic but reduced osteoclastogenic marker expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119164/
https://www.ncbi.nlm.nih.gov/pubmed/32245457
http://dx.doi.org/10.1186/s12906-020-02892-w
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