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Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle
Abnormal neurotransmitter concentration is one of the factors that affect the health status, behavioral personality, and welfare level of animals, but the genetic basis of the abnormality is still largely unknown. The objective of this study is to identify putative genomic loci associated with neuro...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119242/ https://www.ncbi.nlm.nih.gov/pubmed/32292413 http://dx.doi.org/10.3389/fgene.2020.00139 |
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author | Chen, Qiuming Qu, Kaixing Ma, Zhijie Zhan, Jingxi Zhang, Fengwei Shen, Jiafei Ning, Qingqing Jia, Peng Zhang, Jicai Chen, Ningbo Chen, Hong Huang, Bizhi Lei, Chuzhao |
author_facet | Chen, Qiuming Qu, Kaixing Ma, Zhijie Zhan, Jingxi Zhang, Fengwei Shen, Jiafei Ning, Qingqing Jia, Peng Zhang, Jicai Chen, Ningbo Chen, Hong Huang, Bizhi Lei, Chuzhao |
author_sort | Chen, Qiuming |
collection | PubMed |
description | Abnormal neurotransmitter concentration is one of the factors that affect the health status, behavioral personality, and welfare level of animals, but the genetic basis of the abnormality is still largely unknown. The objective of this study is to identify putative genomic loci associated with neurotransmitter concentration in cattle. We measured serotonin (5HT), dopamine (DA), cortisol, glutamate (Glu), and ACTH concentrations in blood serum using double-antibody sandwich ELISA in 30 Brahman cattle and 127 Yunling cattle. Interestingly, we found that ACTH concentration was positively correlated with body weight, cannon circumference, and hip width (P < 0.05). Genome-wide association study (GWAS) was performed with mixed linear models using autosomal SNPs derived from the whole-genome sequence. We identified five, five, two, three, and five suggestive loci associated with 5HT, DA, cortisol, Glu, and ACTH concentration, respectively. These 20 associated loci implicated 18 candidate genes. For Glu concentration, the most significant association locus was assigned to MCHR1, a G-coupled receptor that could modulate glutamate release. For dopamine concentration, a very strong association locus was located in the intron of SLC18A2, which is a critical mediator of dopamine dynamics. However, for ACTH concentration, a very strong association locus was assigned to HTR1F, a G protein-coupled receptor that can influence the release of ACTH. Other candidate genes of interest identified for neurotransmitter concentration were PRMT6, GADD45A, PCCA, ANGPT1, ACCS, LOC100336971, TNR, GSDMA, CNTN3, CARMIL1, CDKAL1, RBFOX1, PCDH15, and LGALS12. Our findings will provide targets for the genetic improvement of neurotransmitter-related traits in domestic cattle and basic materials for studying the mechanism of neurotransmitter synthesis, release, and transport in human and animals. |
format | Online Article Text |
id | pubmed-7119242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71192422020-04-14 Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle Chen, Qiuming Qu, Kaixing Ma, Zhijie Zhan, Jingxi Zhang, Fengwei Shen, Jiafei Ning, Qingqing Jia, Peng Zhang, Jicai Chen, Ningbo Chen, Hong Huang, Bizhi Lei, Chuzhao Front Genet Genetics Abnormal neurotransmitter concentration is one of the factors that affect the health status, behavioral personality, and welfare level of animals, but the genetic basis of the abnormality is still largely unknown. The objective of this study is to identify putative genomic loci associated with neurotransmitter concentration in cattle. We measured serotonin (5HT), dopamine (DA), cortisol, glutamate (Glu), and ACTH concentrations in blood serum using double-antibody sandwich ELISA in 30 Brahman cattle and 127 Yunling cattle. Interestingly, we found that ACTH concentration was positively correlated with body weight, cannon circumference, and hip width (P < 0.05). Genome-wide association study (GWAS) was performed with mixed linear models using autosomal SNPs derived from the whole-genome sequence. We identified five, five, two, three, and five suggestive loci associated with 5HT, DA, cortisol, Glu, and ACTH concentration, respectively. These 20 associated loci implicated 18 candidate genes. For Glu concentration, the most significant association locus was assigned to MCHR1, a G-coupled receptor that could modulate glutamate release. For dopamine concentration, a very strong association locus was located in the intron of SLC18A2, which is a critical mediator of dopamine dynamics. However, for ACTH concentration, a very strong association locus was assigned to HTR1F, a G protein-coupled receptor that can influence the release of ACTH. Other candidate genes of interest identified for neurotransmitter concentration were PRMT6, GADD45A, PCCA, ANGPT1, ACCS, LOC100336971, TNR, GSDMA, CNTN3, CARMIL1, CDKAL1, RBFOX1, PCDH15, and LGALS12. Our findings will provide targets for the genetic improvement of neurotransmitter-related traits in domestic cattle and basic materials for studying the mechanism of neurotransmitter synthesis, release, and transport in human and animals. Frontiers Media S.A. 2020-03-27 /pmc/articles/PMC7119242/ /pubmed/32292413 http://dx.doi.org/10.3389/fgene.2020.00139 Text en Copyright © 2020 Chen, Qu, Ma, Zhan, Zhang, Shen, Ning, Jia, Zhang, Chen, Chen, Huang and Lei. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Chen, Qiuming Qu, Kaixing Ma, Zhijie Zhan, Jingxi Zhang, Fengwei Shen, Jiafei Ning, Qingqing Jia, Peng Zhang, Jicai Chen, Ningbo Chen, Hong Huang, Bizhi Lei, Chuzhao Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title | Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title_full | Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title_fullStr | Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title_full_unstemmed | Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title_short | Genome-Wide Association Study Identifies Genomic Loci Associated With Neurotransmitter Concentration in Cattle |
title_sort | genome-wide association study identifies genomic loci associated with neurotransmitter concentration in cattle |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119242/ https://www.ncbi.nlm.nih.gov/pubmed/32292413 http://dx.doi.org/10.3389/fgene.2020.00139 |
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