Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification

Genomic RNA fingerprints of infectious bronchitis virus (IBV) strains M41 and Conn46 were prepared to identify T1 RNase-resistant oligonucleotides ‘unique’ to each of the two IBV strains. Such oligonucleotides were subsequently eluted from the gels and their nucleotide sequences determined. When oli...

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Detalles Bibliográficos
Autores principales: Karaca, Kemal, Palukaitis, Peter, Naqi, Syed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1993
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119523/
https://www.ncbi.nlm.nih.gov/pubmed/8390475
http://dx.doi.org/10.1016/0166-0934(93)90040-X
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author Karaca, Kemal
Palukaitis, Peter
Naqi, Syed
author_facet Karaca, Kemal
Palukaitis, Peter
Naqi, Syed
author_sort Karaca, Kemal
collection PubMed
description Genomic RNA fingerprints of infectious bronchitis virus (IBV) strains M41 and Conn46 were prepared to identify T1 RNase-resistant oligonucleotides ‘unique’ to each of the two IBV strains. Such oligonucleotides were subsequently eluted from the gels and their nucleotide sequences determined. When oligonucleotide probes of those sequences were synthesized and used in a dot-blot hybridization assay, the probes lacked IBV strain-specificity and reacted with the RNAs of homologous as well as heterologous IBV strains. Based on these results, the methods used in this study need to be applied to a large number of oligonucleotide probes, to find one or a few that might be suitable as IBV strain- or serotype-specific oligonucleotide probes.
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spelling pubmed-71195232020-04-08 Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification Karaca, Kemal Palukaitis, Peter Naqi, Syed J Virol Methods Article Genomic RNA fingerprints of infectious bronchitis virus (IBV) strains M41 and Conn46 were prepared to identify T1 RNase-resistant oligonucleotides ‘unique’ to each of the two IBV strains. Such oligonucleotides were subsequently eluted from the gels and their nucleotide sequences determined. When oligonucleotide probes of those sequences were synthesized and used in a dot-blot hybridization assay, the probes lacked IBV strain-specificity and reacted with the RNAs of homologous as well as heterologous IBV strains. Based on these results, the methods used in this study need to be applied to a large number of oligonucleotide probes, to find one or a few that might be suitable as IBV strain- or serotype-specific oligonucleotide probes. Published by Elsevier B.V. 1993-05 2002-11-12 /pmc/articles/PMC7119523/ /pubmed/8390475 http://dx.doi.org/10.1016/0166-0934(93)90040-X Text en Copyright © 1993 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Karaca, Kemal
Palukaitis, Peter
Naqi, Syed
Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title_full Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title_fullStr Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title_full_unstemmed Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title_short Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
title_sort oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119523/
https://www.ncbi.nlm.nih.gov/pubmed/8390475
http://dx.doi.org/10.1016/0166-0934(93)90040-X
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