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A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus
A solid-phase radioimmunoassay is described for the detection of antibodies to mouse hepatitis virus. Viruses were purified by velocity and isopycnic gradient centrifugation and 96-well plastic plates were coated with viral antigens. To allow the detection of most serotypes of low titered antisera,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1983
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119524/ https://www.ncbi.nlm.nih.gov/pubmed/6308026 http://dx.doi.org/10.1016/0166-0934(83)90040-X |
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author | Leibowitz, J.L. Fung, L.S. Levy, G.A. |
author_facet | Leibowitz, J.L. Fung, L.S. Levy, G.A. |
author_sort | Leibowitz, J.L. |
collection | PubMed |
description | A solid-phase radioimmunoassay is described for the detection of antibodies to mouse hepatitis virus. Viruses were purified by velocity and isopycnic gradient centrifugation and 96-well plastic plates were coated with viral antigens. To allow the detection of most serotypes of low titered antisera, a pool of antigens from several viral serotypes were employed. The second antibody, an affinity-purified goat antimouse immunoglobulin, detects IgG, IgM and IgA antibodies. This assay is more sensitive than either the plaque reduction assay or the commercially available enzyme-linked immunosorbant assay and proved to be useful for screening mouse colonies for the presence of mouse hepatitis virus, following seroconversion in experimental animals and in the production of monoclonal antibodies to both structural and nonstructural proteins. |
format | Online Article Text |
id | pubmed-7119524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71195242020-04-08 A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus Leibowitz, J.L. Fung, L.S. Levy, G.A. J Virol Methods Article A solid-phase radioimmunoassay is described for the detection of antibodies to mouse hepatitis virus. Viruses were purified by velocity and isopycnic gradient centrifugation and 96-well plastic plates were coated with viral antigens. To allow the detection of most serotypes of low titered antisera, a pool of antigens from several viral serotypes were employed. The second antibody, an affinity-purified goat antimouse immunoglobulin, detects IgG, IgM and IgA antibodies. This assay is more sensitive than either the plaque reduction assay or the commercially available enzyme-linked immunosorbant assay and proved to be useful for screening mouse colonies for the presence of mouse hepatitis virus, following seroconversion in experimental animals and in the production of monoclonal antibodies to both structural and nonstructural proteins. Published by Elsevier B.V. 1983-05 2002-11-12 /pmc/articles/PMC7119524/ /pubmed/6308026 http://dx.doi.org/10.1016/0166-0934(83)90040-X Text en Copyright © 1983 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Leibowitz, J.L. Fung, L.S. Levy, G.A. A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title | A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title_full | A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title_fullStr | A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title_full_unstemmed | A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title_short | A sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
title_sort | sensitive radioimmunoassay for the determination of antibodies to mouse hepatitis virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119524/ https://www.ncbi.nlm.nih.gov/pubmed/6308026 http://dx.doi.org/10.1016/0166-0934(83)90040-X |
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