An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells

An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance...

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Detalles Bibliográficos
Autor principal: Smith, Abigail L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1985
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119543/
https://www.ncbi.nlm.nih.gov/pubmed/3932444
http://dx.doi.org/10.1016/0166-0934(85)90025-4
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author Smith, Abigail L.
author_facet Smith, Abigail L.
author_sort Smith, Abigail L.
collection PubMed
description An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance of the enzyme immunoassay. Specificity of the reaction product was shown by neutralization of infectivity using a hyperimmune rabbit antiserum to murine parvovirus.
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spelling pubmed-71195432020-04-08 An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells Smith, Abigail L. J Virol Methods Article An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance of the enzyme immunoassay. Specificity of the reaction product was shown by neutralization of infectivity using a hyperimmune rabbit antiserum to murine parvovirus. Published by Elsevier B.V. 1985-08 2002-11-12 /pmc/articles/PMC7119543/ /pubmed/3932444 http://dx.doi.org/10.1016/0166-0934(85)90025-4 Text en Copyright © 1985 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Smith, Abigail L.
An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title_full An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title_fullStr An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title_full_unstemmed An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title_short An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
title_sort enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119543/
https://www.ncbi.nlm.nih.gov/pubmed/3932444
http://dx.doi.org/10.1016/0166-0934(85)90025-4
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