Characterisation of rubella virus hemagglutinin rosettes

Purified rubella virus treated with Triton X-100 was subjected to centrifugation in a sucrose density gradient containing nonionic detergent β-D-octylglucoside. The result of this treatment was the formation of hemagglutinating rosettes containing viral glycoproteins VP(2) (50,000 mol. wt.) and VP(3...

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Detalles Bibliográficos
Autores principales: Trudel, M., Marchessault, F., Payment, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1981
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119566/
https://www.ncbi.nlm.nih.gov/pubmed/7228976
http://dx.doi.org/10.1016/0166-0934(81)90009-4
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author Trudel, M.
Marchessault, F.
Payment, P.
author_facet Trudel, M.
Marchessault, F.
Payment, P.
author_sort Trudel, M.
collection PubMed
description Purified rubella virus treated with Triton X-100 was subjected to centrifugation in a sucrose density gradient containing nonionic detergent β-D-octylglucoside. The result of this treatment was the formation of hemagglutinating rosettes containing viral glycoproteins VP(2) (50,000 mol. wt.) and VP(3) (63,000 mol. wt.). The rosettes have a 26 S sedimentation coefficient and a density of 1.2 g/cm(3) in sucrose. Electron microscopy revealed 15 nm rosettes with a hollow center. The molecular weight of the rosettes was extrapolated at 850,00
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spelling pubmed-71195662020-04-08 Characterisation of rubella virus hemagglutinin rosettes Trudel, M. Marchessault, F. Payment, P. J Virol Methods Article Purified rubella virus treated with Triton X-100 was subjected to centrifugation in a sucrose density gradient containing nonionic detergent β-D-octylglucoside. The result of this treatment was the formation of hemagglutinating rosettes containing viral glycoproteins VP(2) (50,000 mol. wt.) and VP(3) (63,000 mol. wt.). The rosettes have a 26 S sedimentation coefficient and a density of 1.2 g/cm(3) in sucrose. Electron microscopy revealed 15 nm rosettes with a hollow center. The molecular weight of the rosettes was extrapolated at 850,00 Published by Elsevier B.V. 1981-03 2002-11-13 /pmc/articles/PMC7119566/ /pubmed/7228976 http://dx.doi.org/10.1016/0166-0934(81)90009-4 Text en Copyright © 1981 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Trudel, M.
Marchessault, F.
Payment, P.
Characterisation of rubella virus hemagglutinin rosettes
title Characterisation of rubella virus hemagglutinin rosettes
title_full Characterisation of rubella virus hemagglutinin rosettes
title_fullStr Characterisation of rubella virus hemagglutinin rosettes
title_full_unstemmed Characterisation of rubella virus hemagglutinin rosettes
title_short Characterisation of rubella virus hemagglutinin rosettes
title_sort characterisation of rubella virus hemagglutinin rosettes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119566/
https://www.ncbi.nlm.nih.gov/pubmed/7228976
http://dx.doi.org/10.1016/0166-0934(81)90009-4
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