Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus

The membrane (M) protein of canine coronavirus (CCoV) was cloned and expressed in E. coli. The purified recombinant protein was then evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for detection of CCoV antibodies in dog sera. Fifty serum samples, scree...

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Autores principales: Elia, Gabriella, Fiermonte, Giuseppe, Pratelli, Annamaria, Martella, Vito, Camero, Michele, Cirone, Francesco, Buonavoglia, Canio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119567/
https://www.ncbi.nlm.nih.gov/pubmed/12711056
http://dx.doi.org/10.1016/S0166-0934(03)00064-8
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author Elia, Gabriella
Fiermonte, Giuseppe
Pratelli, Annamaria
Martella, Vito
Camero, Michele
Cirone, Francesco
Buonavoglia, Canio
author_facet Elia, Gabriella
Fiermonte, Giuseppe
Pratelli, Annamaria
Martella, Vito
Camero, Michele
Cirone, Francesco
Buonavoglia, Canio
author_sort Elia, Gabriella
collection PubMed
description The membrane (M) protein of canine coronavirus (CCoV) was cloned and expressed in E. coli. The purified recombinant protein was then evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for detection of CCoV antibodies in dog sera. Fifty serum samples, screened previously by whole virus ELISA and Western blotting, were tested. When the performance of the new test was compared with those of whole virus ELISA and Western blotting, an excellent correlation was found with the latter two assays. The ELISA based on recombinant M protein represents an alternative and valid test for detection of antibodies to CCoV in dog sera.
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spelling pubmed-71195672020-04-08 Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus Elia, Gabriella Fiermonte, Giuseppe Pratelli, Annamaria Martella, Vito Camero, Michele Cirone, Francesco Buonavoglia, Canio J Virol Methods Article The membrane (M) protein of canine coronavirus (CCoV) was cloned and expressed in E. coli. The purified recombinant protein was then evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for detection of CCoV antibodies in dog sera. Fifty serum samples, screened previously by whole virus ELISA and Western blotting, were tested. When the performance of the new test was compared with those of whole virus ELISA and Western blotting, an excellent correlation was found with the latter two assays. The ELISA based on recombinant M protein represents an alternative and valid test for detection of antibodies to CCoV in dog sera. Elsevier Science B.V. 2003-05 2003-04-16 /pmc/articles/PMC7119567/ /pubmed/12711056 http://dx.doi.org/10.1016/S0166-0934(03)00064-8 Text en Copyright © 2003 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Elia, Gabriella
Fiermonte, Giuseppe
Pratelli, Annamaria
Martella, Vito
Camero, Michele
Cirone, Francesco
Buonavoglia, Canio
Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title_full Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title_fullStr Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title_full_unstemmed Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title_short Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus
title_sort recombinant m protein-based elisa test for detection of antibodies to canine coronavirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119567/
https://www.ncbi.nlm.nih.gov/pubmed/12711056
http://dx.doi.org/10.1016/S0166-0934(03)00064-8
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