The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus

An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.

Detalles Bibliográficos
Autores principales: Binns, M.M., Boursnell, M.E.G., Foulds, I.J., Brown, T.D.K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1985
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119584/
https://www.ncbi.nlm.nih.gov/pubmed/2993338
http://dx.doi.org/10.1016/0166-0934(85)90116-8
Descripción
Sumario:An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.

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