The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus

An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.

Detalles Bibliográficos
Autores principales: Binns, M.M., Boursnell, M.E.G., Foulds, I.J., Brown, T.D.K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1985
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119584/
https://www.ncbi.nlm.nih.gov/pubmed/2993338
http://dx.doi.org/10.1016/0166-0934(85)90116-8
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author Binns, M.M.
Boursnell, M.E.G.
Foulds, I.J.
Brown, T.D.K.
author_facet Binns, M.M.
Boursnell, M.E.G.
Foulds, I.J.
Brown, T.D.K.
author_sort Binns, M.M.
collection PubMed
description An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.
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spelling pubmed-71195842020-04-08 The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus Binns, M.M. Boursnell, M.E.G. Foulds, I.J. Brown, T.D.K. J Virol Methods Article An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences. Published by Elsevier B.V. 1985-07 2002-11-12 /pmc/articles/PMC7119584/ /pubmed/2993338 http://dx.doi.org/10.1016/0166-0934(85)90116-8 Text en Copyright © 1985 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Binns, M.M.
Boursnell, M.E.G.
Foulds, I.J.
Brown, T.D.K.
The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title_full The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title_fullStr The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title_full_unstemmed The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title_short The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus
title_sort use of a random priming procedure to generate cdna libraries of infectious bronchitis virus, a large rna virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119584/
https://www.ncbi.nlm.nih.gov/pubmed/2993338
http://dx.doi.org/10.1016/0166-0934(85)90116-8
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