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Cloning, expression and characterization of biologically active feline tumour necrosis factor-α
We report the cloning, expression and characterization of biologically active feline tumour necrosis factor-α (fTNF-α). Messenger RNA was extracted from feline peritoneal macrophage cultures and used to synthesize cDNA for polymerase chain reaction (PCR) amplification. The PCR products were cloned i...
Autores principales: | Rimstad, Espen, Reubel, Gerhard H., Dean, Gregg A., Higgins, Joanne, Pedersen, Niels C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119607/ https://www.ncbi.nlm.nih.gov/pubmed/7676612 http://dx.doi.org/10.1016/0165-2427(94)05345-S |
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