Two enzyme immunoassays for the detection of antibody to rodent coronaviruses

Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strai...

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Detalles Bibliográficos
Autores principales: Smith, Abigail L., Winograd, Deborah F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1986
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119637/
https://www.ncbi.nlm.nih.gov/pubmed/3025244
http://dx.doi.org/10.1016/0166-0934(86)90035-2
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author Smith, Abigail L.
Winograd, Deborah F.
author_facet Smith, Abigail L.
Winograd, Deborah F.
author_sort Smith, Abigail L.
collection PubMed
description Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.
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spelling pubmed-71196372020-04-08 Two enzyme immunoassays for the detection of antibody to rodent coronaviruses Smith, Abigail L. Winograd, Deborah F. J Virol Methods Article Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months. Published by Elsevier B.V. 1986-11 2002-11-12 /pmc/articles/PMC7119637/ /pubmed/3025244 http://dx.doi.org/10.1016/0166-0934(86)90035-2 Text en Copyright © 1986 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Smith, Abigail L.
Winograd, Deborah F.
Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title_full Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title_fullStr Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title_full_unstemmed Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title_short Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
title_sort two enzyme immunoassays for the detection of antibody to rodent coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119637/
https://www.ncbi.nlm.nih.gov/pubmed/3025244
http://dx.doi.org/10.1016/0166-0934(86)90035-2
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