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The use of Armored RNA as a multi-purpose internal control for RT-PCR
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119664/ https://www.ncbi.nlm.nih.gov/pubmed/18395804 http://dx.doi.org/10.1016/j.jviromet.2008.02.007 |
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author | Stevenson, Jeffery Hymas, Weston Hillyard, David |
author_facet | Stevenson, Jeffery Hymas, Weston Hillyard, David |
author_sort | Stevenson, Jeffery |
collection | PubMed |
description | Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA. |
format | Online Article Text |
id | pubmed-7119664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71196642020-04-08 The use of Armored RNA as a multi-purpose internal control for RT-PCR Stevenson, Jeffery Hymas, Weston Hillyard, David J Virol Methods Article Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA. Elsevier B.V. 2008-06 2008-04-18 /pmc/articles/PMC7119664/ /pubmed/18395804 http://dx.doi.org/10.1016/j.jviromet.2008.02.007 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Stevenson, Jeffery Hymas, Weston Hillyard, David The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title | The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title_full | The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title_fullStr | The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title_full_unstemmed | The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title_short | The use of Armored RNA as a multi-purpose internal control for RT-PCR |
title_sort | use of armored rna as a multi-purpose internal control for rt-pcr |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119664/ https://www.ncbi.nlm.nih.gov/pubmed/18395804 http://dx.doi.org/10.1016/j.jviromet.2008.02.007 |
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