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Growth of a murine coronavirus in a microcarrier cell culture system
The growth of the murine coronavirus MHV-A59 on murine DBT cells adapted to dextran-made Cytodex 1 microcarriers was studied in comparison with cells grown on plastic dishes. With a microcarrier concentration of 5 g/l in spinner flasks, a density of 3 × 10(6) cells/ml was reached in 7 days. Under th...
Autores principales: | Talbot, Pierre J., Lapierre, Jacques, Daniel, Claude, Dugré, Robert, Trépanier, Pierre |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1989
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119676/ https://www.ncbi.nlm.nih.gov/pubmed/2476458 http://dx.doi.org/10.1016/0166-0934(89)90100-6 |
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