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Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?

Rapid diagnosis of adenoviral respiratory infections is required in order to decide optimal treatment strategies. The adenovirus antigen immunochromatography Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain), was evaluated versus the shell-vial culture and multiplex PCR (Clart Pne...

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Autores principales: Romero-Gómez, María Pilar, López, Rosario López, Montes, Remedios González, Ruiz, Cristina Ots, Cuesta, Sara Hierro, Crespo, María Antonia Martín, García, Santos García
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119683/
https://www.ncbi.nlm.nih.gov/pubmed/24096271
http://dx.doi.org/10.1016/j.jviromet.2013.09.002
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author Romero-Gómez, María Pilar
López, Rosario López
Montes, Remedios González
Ruiz, Cristina Ots
Cuesta, Sara Hierro
Crespo, María Antonia Martín
García, Santos García
author_facet Romero-Gómez, María Pilar
López, Rosario López
Montes, Remedios González
Ruiz, Cristina Ots
Cuesta, Sara Hierro
Crespo, María Antonia Martín
García, Santos García
author_sort Romero-Gómez, María Pilar
collection PubMed
description Rapid diagnosis of adenoviral respiratory infections is required in order to decide optimal treatment strategies. The adenovirus antigen immunochromatography Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain), was evaluated versus the shell-vial culture and multiplex PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain), in nasopharyngeal washes and oropharyngeal swabs specimens from subjects with respiratory tract infections. Between April 2011 and November 2012, 224 patients were included. The IC Adeno Respiratory Card Letitest was positive for 77.9% (74 of 95) of patients diagnosed at bedside. Using multiplex-PCR as the reference standard, the overall sensitivity was 77.9% and the specificity was 73.6%. Taking shell-viral culture as the reference method, the Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) sensitivity and specificity values were 80.0% and 60.9%, respectively. Using RT-PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain) as the reference standard, the viral culture sensitivity was 53.2% and the specificity was 100%. The Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) assay could be a simple and rapid method for antigenic detection of adenovirus in pediatric respiratory samples although it would be necessary to improve the specificity and sensitivity of the test.
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spelling pubmed-71196832020-04-08 Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department? Romero-Gómez, María Pilar López, Rosario López Montes, Remedios González Ruiz, Cristina Ots Cuesta, Sara Hierro Crespo, María Antonia Martín García, Santos García J Virol Methods Article Rapid diagnosis of adenoviral respiratory infections is required in order to decide optimal treatment strategies. The adenovirus antigen immunochromatography Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain), was evaluated versus the shell-vial culture and multiplex PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain), in nasopharyngeal washes and oropharyngeal swabs specimens from subjects with respiratory tract infections. Between April 2011 and November 2012, 224 patients were included. The IC Adeno Respiratory Card Letitest was positive for 77.9% (74 of 95) of patients diagnosed at bedside. Using multiplex-PCR as the reference standard, the overall sensitivity was 77.9% and the specificity was 73.6%. Taking shell-viral culture as the reference method, the Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) sensitivity and specificity values were 80.0% and 60.9%, respectively. Using RT-PCR (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain) as the reference standard, the viral culture sensitivity was 53.2% and the specificity was 100%. The Adeno Respiratory Card Letitest (Leti diagnostics, Barcelona, Spain) assay could be a simple and rapid method for antigenic detection of adenovirus in pediatric respiratory samples although it would be necessary to improve the specificity and sensitivity of the test. Elsevier B.V. 2014-01 2013-10-03 /pmc/articles/PMC7119683/ /pubmed/24096271 http://dx.doi.org/10.1016/j.jviromet.2013.09.002 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Romero-Gómez, María Pilar
López, Rosario López
Montes, Remedios González
Ruiz, Cristina Ots
Cuesta, Sara Hierro
Crespo, María Antonia Martín
García, Santos García
Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title_full Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title_fullStr Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title_full_unstemmed Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title_short Immunochromatographic test for detection of adenovirus from respiratory samples: Is it a real solution for pediatric emergency department?
title_sort immunochromatographic test for detection of adenovirus from respiratory samples: is it a real solution for pediatric emergency department?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119683/
https://www.ncbi.nlm.nih.gov/pubmed/24096271
http://dx.doi.org/10.1016/j.jviromet.2013.09.002
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