A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than...

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Detalles Bibliográficos
Autores principales: Xia, Hongyan, Liu, Lihong, Nordengrahn, Ann, Kiss, István, Merza, Malik, Eriksson, Ronnie, Blomberg, Jonas, Belák, Sándor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119709/
https://www.ncbi.nlm.nih.gov/pubmed/20403384
http://dx.doi.org/10.1016/j.jviromet.2010.04.009
Descripción
Sumario:This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.

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