A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than...

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Autores principales: Xia, Hongyan, Liu, Lihong, Nordengrahn, Ann, Kiss, István, Merza, Malik, Eriksson, Ronnie, Blomberg, Jonas, Belák, Sándor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119709/
https://www.ncbi.nlm.nih.gov/pubmed/20403384
http://dx.doi.org/10.1016/j.jviromet.2010.04.009
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author Xia, Hongyan
Liu, Lihong
Nordengrahn, Ann
Kiss, István
Merza, Malik
Eriksson, Ronnie
Blomberg, Jonas
Belák, Sándor
author_facet Xia, Hongyan
Liu, Lihong
Nordengrahn, Ann
Kiss, István
Merza, Malik
Eriksson, Ronnie
Blomberg, Jonas
Belák, Sándor
author_sort Xia, Hongyan
collection PubMed
description This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.
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spelling pubmed-71197092020-04-08 A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies Xia, Hongyan Liu, Lihong Nordengrahn, Ann Kiss, István Merza, Malik Eriksson, Ronnie Blomberg, Jonas Belák, Sándor J Virol Methods Article This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. Elsevier B.V. 2010-09 2010-04-18 /pmc/articles/PMC7119709/ /pubmed/20403384 http://dx.doi.org/10.1016/j.jviromet.2010.04.009 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Xia, Hongyan
Liu, Lihong
Nordengrahn, Ann
Kiss, István
Merza, Malik
Eriksson, Ronnie
Blomberg, Jonas
Belák, Sándor
A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title_full A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title_fullStr A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title_full_unstemmed A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title_short A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
title_sort microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119709/
https://www.ncbi.nlm.nih.gov/pubmed/20403384
http://dx.doi.org/10.1016/j.jviromet.2010.04.009
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