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Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease

The early stages of the host response to infectious agents include a number of physiologic changes, collectively known as the acute phase response. The acute phase response is comprised of reactions localized at the site of infection, as well as the initiation of systemic responses, which include a...

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Autores principales: Godson, Dale L., Campos, Manuel, Attah-Poku, Samuel K., Redmond, Mark J., Cordeiro, Daphne M., Sethi, Manjeet S., Harland, Richard J., Babiuk, Lorne A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119716/
https://www.ncbi.nlm.nih.gov/pubmed/8792565
http://dx.doi.org/10.1016/0165-2427(95)05520-7
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author Godson, Dale L.
Campos, Manuel
Attah-Poku, Samuel K.
Redmond, Mark J.
Cordeiro, Daphne M.
Sethi, Manjeet S.
Harland, Richard J.
Babiuk, Lorne A.
author_facet Godson, Dale L.
Campos, Manuel
Attah-Poku, Samuel K.
Redmond, Mark J.
Cordeiro, Daphne M.
Sethi, Manjeet S.
Harland, Richard J.
Babiuk, Lorne A.
author_sort Godson, Dale L.
collection PubMed
description The early stages of the host response to infectious agents include a number of physiologic changes, collectively known as the acute phase response. The acute phase response is comprised of reactions localized at the site of infection, as well as the initiation of systemic responses, which include a rapid increase in the serum concentration of some proteins, known as acute phase proteins (APP). Using polyacrylamide gel electrophoresis, we detected two APP of approximately 22 and 37 kDa molecular weight in sera obtained from cattle with bovine respiratory disease (BRD). Based on their presence in the sera of sick, but not normal animals, the molecular weights, N-terminal amino acid sequence analysis, and the ability to bind hemoglobin, we identified these proteins as the α and β subunits of haptoglobin. The haptoglobin molecule and the α subunit were isolated from serum, purified, and used to produce monoclonal and polyclonal antibodies. With these reagents, an enzyme linked immunosorbent assay was developed to measure the concentration of haptoglobin in bovine serum. Using an experimental model of BRD induced by a sequential challenge of calves with bovine herpesvirus type-1 and Pasteurella haemolytica, we observed a temporal relationship between the increase in haptoglobin concentration in serum and the onset of bacterial infection. The haptoglobin concentration ranged from undetectable in the serum of most calves prior to challenge, to greater than 1 mgml(−1) in over one-third of the calves at the height of disease. Furthermore, the concentration of haptoglobin was associated significantly with other measures of the severity of disease. Together, these results indicate that quantification of acute phase proteins in animals with BRD could be a valuable diagnostic and prognostic aid.
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spelling pubmed-71197162020-04-08 Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease Godson, Dale L. Campos, Manuel Attah-Poku, Samuel K. Redmond, Mark J. Cordeiro, Daphne M. Sethi, Manjeet S. Harland, Richard J. Babiuk, Lorne A. Vet Immunol Immunopathol Article The early stages of the host response to infectious agents include a number of physiologic changes, collectively known as the acute phase response. The acute phase response is comprised of reactions localized at the site of infection, as well as the initiation of systemic responses, which include a rapid increase in the serum concentration of some proteins, known as acute phase proteins (APP). Using polyacrylamide gel electrophoresis, we detected two APP of approximately 22 and 37 kDa molecular weight in sera obtained from cattle with bovine respiratory disease (BRD). Based on their presence in the sera of sick, but not normal animals, the molecular weights, N-terminal amino acid sequence analysis, and the ability to bind hemoglobin, we identified these proteins as the α and β subunits of haptoglobin. The haptoglobin molecule and the α subunit were isolated from serum, purified, and used to produce monoclonal and polyclonal antibodies. With these reagents, an enzyme linked immunosorbent assay was developed to measure the concentration of haptoglobin in bovine serum. Using an experimental model of BRD induced by a sequential challenge of calves with bovine herpesvirus type-1 and Pasteurella haemolytica, we observed a temporal relationship between the increase in haptoglobin concentration in serum and the onset of bacterial infection. The haptoglobin concentration ranged from undetectable in the serum of most calves prior to challenge, to greater than 1 mgml(−1) in over one-third of the calves at the height of disease. Furthermore, the concentration of haptoglobin was associated significantly with other measures of the severity of disease. Together, these results indicate that quantification of acute phase proteins in animals with BRD could be a valuable diagnostic and prognostic aid. Published by Elsevier B.V. 1996-06-01 1999-03-08 /pmc/articles/PMC7119716/ /pubmed/8792565 http://dx.doi.org/10.1016/0165-2427(95)05520-7 Text en Copyright © 1996 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Godson, Dale L.
Campos, Manuel
Attah-Poku, Samuel K.
Redmond, Mark J.
Cordeiro, Daphne M.
Sethi, Manjeet S.
Harland, Richard J.
Babiuk, Lorne A.
Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title_full Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title_fullStr Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title_full_unstemmed Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title_short Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
title_sort serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119716/
https://www.ncbi.nlm.nih.gov/pubmed/8792565
http://dx.doi.org/10.1016/0165-2427(95)05520-7
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