Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay

The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result o...

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Autores principales: Lugovskaya, N.N., Scherbakov, A.V., Yakovleva, A.S., Tsyvanyuk, M.A., Mudrak, N.S., Drygin, V.V., Borisov, A.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119718/
https://www.ncbi.nlm.nih.gov/pubmed/16675032
http://dx.doi.org/10.1016/j.jviromet.2006.03.019
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author Lugovskaya, N.N.
Scherbakov, A.V.
Yakovleva, A.S.
Tsyvanyuk, M.A.
Mudrak, N.S.
Drygin, V.V.
Borisov, A.V.
author_facet Lugovskaya, N.N.
Scherbakov, A.V.
Yakovleva, A.S.
Tsyvanyuk, M.A.
Mudrak, N.S.
Drygin, V.V.
Borisov, A.V.
author_sort Lugovskaya, N.N.
collection PubMed
description The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.
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spelling pubmed-71197182020-04-08 Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay Lugovskaya, N.N. Scherbakov, A.V. Yakovleva, A.S. Tsyvanyuk, M.A. Mudrak, N.S. Drygin, V.V. Borisov, A.V. J Virol Methods Article The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%. Elsevier B.V. 2006-08 2006-05-03 /pmc/articles/PMC7119718/ /pubmed/16675032 http://dx.doi.org/10.1016/j.jviromet.2006.03.019 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lugovskaya, N.N.
Scherbakov, A.V.
Yakovleva, A.S.
Tsyvanyuk, M.A.
Mudrak, N.S.
Drygin, V.V.
Borisov, A.V.
Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title_full Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title_fullStr Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title_full_unstemmed Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title_short Detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
title_sort detection of antibodies to avian infectious bronchitis virus by a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119718/
https://www.ncbi.nlm.nih.gov/pubmed/16675032
http://dx.doi.org/10.1016/j.jviromet.2006.03.019
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