Cargando…

Development of an RT-PCR diagnostic test for an avian astrovirus

Astroviruses are small round viruses that cause enteric disease in the young of several species. Detection and diagnosis of astrovirus infection in non-human hosts relies heavily on electron microscopy and fluorescent antibody tests. Recently, our laboratory isolated and sequenced an avian astroviru...

Descripción completa

Detalles Bibliográficos
Autores principales: Koci, Matthew D, Seal, Bruce S, Schultz-Cherry, Stacey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119769/
https://www.ncbi.nlm.nih.gov/pubmed/11011083
http://dx.doi.org/10.1016/S0166-0934(00)00228-7
_version_ 1783514831547006976
author Koci, Matthew D
Seal, Bruce S
Schultz-Cherry, Stacey
author_facet Koci, Matthew D
Seal, Bruce S
Schultz-Cherry, Stacey
author_sort Koci, Matthew D
collection PubMed
description Astroviruses are small round viruses that cause enteric disease in the young of several species. Detection and diagnosis of astrovirus infection in non-human hosts relies heavily on electron microscopy and fluorescent antibody tests. Recently, our laboratory isolated and sequenced an avian astrovirus from poult enteritis mortality syndrome affected turkeys. These studies describe the development of RT-PCR methods, which specifically detect regions of the viral capsid and polymerase genes, and demonstrate their use in detecting astrovirus infection in commercial turkey flocks.
format Online
Article
Text
id pubmed-7119769
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher Elsevier Science B.V.
record_format MEDLINE/PubMed
spelling pubmed-71197692020-04-08 Development of an RT-PCR diagnostic test for an avian astrovirus Koci, Matthew D Seal, Bruce S Schultz-Cherry, Stacey J Virol Methods Article Astroviruses are small round viruses that cause enteric disease in the young of several species. Detection and diagnosis of astrovirus infection in non-human hosts relies heavily on electron microscopy and fluorescent antibody tests. Recently, our laboratory isolated and sequenced an avian astrovirus from poult enteritis mortality syndrome affected turkeys. These studies describe the development of RT-PCR methods, which specifically detect regions of the viral capsid and polymerase genes, and demonstrate their use in detecting astrovirus infection in commercial turkey flocks. Elsevier Science B.V. 2000-10 2000-09-25 /pmc/articles/PMC7119769/ /pubmed/11011083 http://dx.doi.org/10.1016/S0166-0934(00)00228-7 Text en Copyright © 2000 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Koci, Matthew D
Seal, Bruce S
Schultz-Cherry, Stacey
Development of an RT-PCR diagnostic test for an avian astrovirus
title Development of an RT-PCR diagnostic test for an avian astrovirus
title_full Development of an RT-PCR diagnostic test for an avian astrovirus
title_fullStr Development of an RT-PCR diagnostic test for an avian astrovirus
title_full_unstemmed Development of an RT-PCR diagnostic test for an avian astrovirus
title_short Development of an RT-PCR diagnostic test for an avian astrovirus
title_sort development of an rt-pcr diagnostic test for an avian astrovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119769/
https://www.ncbi.nlm.nih.gov/pubmed/11011083
http://dx.doi.org/10.1016/S0166-0934(00)00228-7
work_keys_str_mv AT kocimatthewd developmentofanrtpcrdiagnostictestforanavianastrovirus
AT sealbruces developmentofanrtpcrdiagnostictestforanavianastrovirus
AT schultzcherrystacey developmentofanrtpcrdiagnostictestforanavianastrovirus