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An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Published by Elsevier B.V.
1986
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119784/ https://www.ncbi.nlm.nih.gov/pubmed/3025238 http://dx.doi.org/10.1016/0166-0934(86)90024-8 |
| Sumario: | Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 × g, 2,100 × g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour. |
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