An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy

Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low...

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Detalles Bibliográficos
Autores principales: Codd, A.A., Narang, H.K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1986
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119784/
https://www.ncbi.nlm.nih.gov/pubmed/3025238
http://dx.doi.org/10.1016/0166-0934(86)90024-8
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author Codd, A.A.
Narang, H.K.
author_facet Codd, A.A.
Narang, H.K.
author_sort Codd, A.A.
collection PubMed
description Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 × g, 2,100 × g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour.
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spelling pubmed-71197842020-04-08 An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy Codd, A.A. Narang, H.K. J Virol Methods Article Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 × g, 2,100 × g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour. Published by Elsevier B.V. 1986-11 2002-11-12 /pmc/articles/PMC7119784/ /pubmed/3025238 http://dx.doi.org/10.1016/0166-0934(86)90024-8 Text en Copyright © 1986 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Codd, A.A.
Narang, H.K.
An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title_full An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title_fullStr An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title_full_unstemmed An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title_short An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
title_sort ion-exchange capture technique for routine identification of faecal viruses by electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119784/
https://www.ncbi.nlm.nih.gov/pubmed/3025238
http://dx.doi.org/10.1016/0166-0934(86)90024-8
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