Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6

Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-...

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Detalles Bibliográficos
Autores principales: Daniel, Claude, Lamarre, Alain, Talbot, Pierre J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1994
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119785/
https://www.ncbi.nlm.nih.gov/pubmed/7714047
http://dx.doi.org/10.1016/0166-0934(94)90180-5
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author Daniel, Claude
Lamarre, Alain
Talbot, Pierre J.
author_facet Daniel, Claude
Lamarre, Alain
Talbot, Pierre J.
author_sort Daniel, Claude
collection PubMed
description Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.
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spelling pubmed-71197852020-04-08 Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6 Daniel, Claude Lamarre, Alain Talbot, Pierre J. J Virol Methods Article Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers. Published by Elsevier B.V. 1994-12 2002-12-10 /pmc/articles/PMC7119785/ /pubmed/7714047 http://dx.doi.org/10.1016/0166-0934(94)90180-5 Text en Copyright © 1994 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Daniel, Claude
Lamarre, Alain
Talbot, Pierre J.
Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title_full Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title_fullStr Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title_full_unstemmed Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title_short Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
title_sort increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at ph 6
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119785/
https://www.ncbi.nlm.nih.gov/pubmed/7714047
http://dx.doi.org/10.1016/0166-0934(94)90180-5
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