Cargando…
An updated TaqMan real-time PCR for canine and feline parvoviruses
Canine parvovirus type 2 (CPV-2) emerged in late 1970s from the feline panleukopenia virus (FPLV) and developed, since then, into novel genetic and antigenic variants (CPV-2a, -2b and -2c). Canine and feline parvoviruses cause an acute enteric disease in their hosts, with high level of viral sheddin...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119788/ https://www.ncbi.nlm.nih.gov/pubmed/23680092 http://dx.doi.org/10.1016/j.jviromet.2013.04.025 |
_version_ | 1783514834380259328 |
---|---|
author | Streck, André Felipe Rüster, Dana Truyen, Uwe Homeier, Timo |
author_facet | Streck, André Felipe Rüster, Dana Truyen, Uwe Homeier, Timo |
author_sort | Streck, André Felipe |
collection | PubMed |
description | Canine parvovirus type 2 (CPV-2) emerged in late 1970s from the feline panleukopenia virus (FPLV) and developed, since then, into novel genetic and antigenic variants (CPV-2a, -2b and -2c). Canine and feline parvoviruses cause an acute enteric disease in their hosts, with high level of viral shedding. In this study, a quantitative TaqMan PCR for detection and quantitation of canine and feline parvoviruses in serum and fecal samples was developed. The primers were designed based upon the entire GenBank content for CPV and FPLV. A standard curve was generated, and validation tests were performed using 10-fold serial dilutions of CPV-2 virus in CPV/FPLV-negative feces and CPV/FPLV-negative serum samples. As a result, the 100% detection limit of the PCR was 18 copies of the viral genome per μl of serum and fecal sample. All canine parvovirus types as well as FPLV were detected. In conclusion, the real-time PCR represents an upgraded and useful tool to identify and quantify canine and feline parvoviruses in different sample matrices. |
format | Online Article Text |
id | pubmed-7119788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71197882020-04-08 An updated TaqMan real-time PCR for canine and feline parvoviruses Streck, André Felipe Rüster, Dana Truyen, Uwe Homeier, Timo J Virol Methods Short Communication Canine parvovirus type 2 (CPV-2) emerged in late 1970s from the feline panleukopenia virus (FPLV) and developed, since then, into novel genetic and antigenic variants (CPV-2a, -2b and -2c). Canine and feline parvoviruses cause an acute enteric disease in their hosts, with high level of viral shedding. In this study, a quantitative TaqMan PCR for detection and quantitation of canine and feline parvoviruses in serum and fecal samples was developed. The primers were designed based upon the entire GenBank content for CPV and FPLV. A standard curve was generated, and validation tests were performed using 10-fold serial dilutions of CPV-2 virus in CPV/FPLV-negative feces and CPV/FPLV-negative serum samples. As a result, the 100% detection limit of the PCR was 18 copies of the viral genome per μl of serum and fecal sample. All canine parvovirus types as well as FPLV were detected. In conclusion, the real-time PCR represents an upgraded and useful tool to identify and quantify canine and feline parvoviruses in different sample matrices. Elsevier B.V. 2013-10 2013-05-13 /pmc/articles/PMC7119788/ /pubmed/23680092 http://dx.doi.org/10.1016/j.jviromet.2013.04.025 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Streck, André Felipe Rüster, Dana Truyen, Uwe Homeier, Timo An updated TaqMan real-time PCR for canine and feline parvoviruses |
title | An updated TaqMan real-time PCR for canine and feline parvoviruses |
title_full | An updated TaqMan real-time PCR for canine and feline parvoviruses |
title_fullStr | An updated TaqMan real-time PCR for canine and feline parvoviruses |
title_full_unstemmed | An updated TaqMan real-time PCR for canine and feline parvoviruses |
title_short | An updated TaqMan real-time PCR for canine and feline parvoviruses |
title_sort | updated taqman real-time pcr for canine and feline parvoviruses |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119788/ https://www.ncbi.nlm.nih.gov/pubmed/23680092 http://dx.doi.org/10.1016/j.jviromet.2013.04.025 |
work_keys_str_mv | AT streckandrefelipe anupdatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT rusterdana anupdatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT truyenuwe anupdatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT homeiertimo anupdatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT streckandrefelipe updatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT rusterdana updatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT truyenuwe updatedtaqmanrealtimepcrforcanineandfelineparvoviruses AT homeiertimo updatedtaqmanrealtimepcrforcanineandfelineparvoviruses |