PCR assay for the detection and the identification of atypical canine coronavirus in dogs

Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitu...

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Detalles Bibliográficos
Autores principales: Pratelli, Annamaria, Tinelli, Antonella, Decaro, Nicola, Camero, Michele, Elia, Gabriella, Gentile, Arturo, Buonavoglia, Canio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119860/
https://www.ncbi.nlm.nih.gov/pubmed/12393151
http://dx.doi.org/10.1016/S0166-0934(02)00165-9
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author Pratelli, Annamaria
Tinelli, Antonella
Decaro, Nicola
Camero, Michele
Elia, Gabriella
Gentile, Arturo
Buonavoglia, Canio
author_facet Pratelli, Annamaria
Tinelli, Antonella
Decaro, Nicola
Camero, Michele
Elia, Gabriella
Gentile, Arturo
Buonavoglia, Canio
author_sort Pratelli, Annamaria
collection PubMed
description Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitutions in the M gene. A total of 177 faecal samples from dogs CCoV positive previously with the PCR assay were analysed. Sixty-two of the 177 samples were amplified with the PCR described in the present study and were thus considered atypical CCoVs. The specificity of the PCR typing assay was confirmed by sequence analysis of the PCR products.
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spelling pubmed-71198602020-04-08 PCR assay for the detection and the identification of atypical canine coronavirus in dogs Pratelli, Annamaria Tinelli, Antonella Decaro, Nicola Camero, Michele Elia, Gabriella Gentile, Arturo Buonavoglia, Canio J Virol Methods Article Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitutions in the M gene. A total of 177 faecal samples from dogs CCoV positive previously with the PCR assay were analysed. Sixty-two of the 177 samples were amplified with the PCR described in the present study and were thus considered atypical CCoVs. The specificity of the PCR typing assay was confirmed by sequence analysis of the PCR products. Elsevier Science B.V. 2002-12 2002-10-01 /pmc/articles/PMC7119860/ /pubmed/12393151 http://dx.doi.org/10.1016/S0166-0934(02)00165-9 Text en Copyright © 2002 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pratelli, Annamaria
Tinelli, Antonella
Decaro, Nicola
Camero, Michele
Elia, Gabriella
Gentile, Arturo
Buonavoglia, Canio
PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title_full PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title_fullStr PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title_full_unstemmed PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title_short PCR assay for the detection and the identification of atypical canine coronavirus in dogs
title_sort pcr assay for the detection and the identification of atypical canine coronavirus in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119860/
https://www.ncbi.nlm.nih.gov/pubmed/12393151
http://dx.doi.org/10.1016/S0166-0934(02)00165-9
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