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Quantitative real-time PCR for the measurement of feline cytokine mRNA

We have developed real-time PCR systems to quantitate feline cytokine gene expression. The method is based on the cleavage of fluorescent dye-labelled probes by the 5′–3′ exonuclease activity of the Taq DNA polymerase during PCR and measurement of fluorescence intensity by a Sequence Detection Syste...

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Detalles Bibliográficos
Autores principales:	Leutenegger, Christian M, Mislin, Caroline N, Sigrist, Brigitte, Ehrengruber, Markus U, Hofmann-Lehmann, Regina, Lutz, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Science B.V. 1999
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119904/ https://www.ncbi.nlm.nih.gov/pubmed/10587308 http://dx.doi.org/10.1016/S0165-2427(99)00100-2

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