Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins

Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliabi...

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Detalles Bibliográficos
Autores principales: Stellberger, Thorsten, Häuser, Roman, Uetz, Peter, von Brunn, Albrecht
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120008/
https://www.ncbi.nlm.nih.gov/pubmed/22130999
http://dx.doi.org/10.1007/978-1-61779-424-7_21
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author Stellberger, Thorsten
Häuser, Roman
Uetz, Peter
von Brunn, Albrecht
author_facet Stellberger, Thorsten
Häuser, Roman
Uetz, Peter
von Brunn, Albrecht
author_sort Stellberger, Thorsten
collection PubMed
description Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.
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spelling pubmed-71200082020-04-06 Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins Stellberger, Thorsten Häuser, Roman Uetz, Peter von Brunn, Albrecht Functional Genomics Article Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system. 2011-07-26 /pmc/articles/PMC7120008/ /pubmed/22130999 http://dx.doi.org/10.1007/978-1-61779-424-7_21 Text en © Springer Science+Business Media, LLC 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Stellberger, Thorsten
Häuser, Roman
Uetz, Peter
von Brunn, Albrecht
Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title_full Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title_fullStr Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title_full_unstemmed Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title_short Yeast Two-Hybrid Screens: Improvement of Array-Based Screening Results by N- and C-terminally Tagged Fusion Proteins
title_sort yeast two-hybrid screens: improvement of array-based screening results by n- and c-terminally tagged fusion proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120008/
https://www.ncbi.nlm.nih.gov/pubmed/22130999
http://dx.doi.org/10.1007/978-1-61779-424-7_21
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