Cargando…

Real-Time RT-PCR Detection of Equine Coronavirus

Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in o...

Descripción completa

Detalles Bibliográficos
Autores principales: Miszczak, Fabien, Kin, Nathalie, Tesson, Vincent, Vabret, Astrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120059/
http://dx.doi.org/10.1007/978-1-4939-3414-0_8
_version_ 1783514892341346304
author Miszczak, Fabien
Kin, Nathalie
Tesson, Vincent
Vabret, Astrid
author_facet Miszczak, Fabien
Kin, Nathalie
Tesson, Vincent
Vabret, Astrid
author_sort Miszczak, Fabien
collection PubMed
description Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples.
format Online
Article
Text
id pubmed-7120059
institution National Center for Biotechnology Information
language English
publishDate 2015
record_format MEDLINE/PubMed
spelling pubmed-71200592020-04-06 Real-Time RT-PCR Detection of Equine Coronavirus Miszczak, Fabien Kin, Nathalie Tesson, Vincent Vabret, Astrid Animal Coronaviruses Article Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples. 2015-09-10 /pmc/articles/PMC7120059/ http://dx.doi.org/10.1007/978-1-4939-3414-0_8 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Miszczak, Fabien
Kin, Nathalie
Tesson, Vincent
Vabret, Astrid
Real-Time RT-PCR Detection of Equine Coronavirus
title Real-Time RT-PCR Detection of Equine Coronavirus
title_full Real-Time RT-PCR Detection of Equine Coronavirus
title_fullStr Real-Time RT-PCR Detection of Equine Coronavirus
title_full_unstemmed Real-Time RT-PCR Detection of Equine Coronavirus
title_short Real-Time RT-PCR Detection of Equine Coronavirus
title_sort real-time rt-pcr detection of equine coronavirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120059/
http://dx.doi.org/10.1007/978-1-4939-3414-0_8
work_keys_str_mv AT miszczakfabien realtimertpcrdetectionofequinecoronavirus
AT kinnathalie realtimertpcrdetectionofequinecoronavirus
AT tessonvincent realtimertpcrdetectionofequinecoronavirus
AT vabretastrid realtimertpcrdetectionofequinecoronavirus