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Real-Time RT-PCR Detection of Equine Coronavirus
Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120059/ http://dx.doi.org/10.1007/978-1-4939-3414-0_8 |
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author | Miszczak, Fabien Kin, Nathalie Tesson, Vincent Vabret, Astrid |
author_facet | Miszczak, Fabien Kin, Nathalie Tesson, Vincent Vabret, Astrid |
author_sort | Miszczak, Fabien |
collection | PubMed |
description | Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples. |
format | Online Article Text |
id | pubmed-7120059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71200592020-04-06 Real-Time RT-PCR Detection of Equine Coronavirus Miszczak, Fabien Kin, Nathalie Tesson, Vincent Vabret, Astrid Animal Coronaviruses Article Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and respiratory samples. 2015-09-10 /pmc/articles/PMC7120059/ http://dx.doi.org/10.1007/978-1-4939-3414-0_8 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Miszczak, Fabien Kin, Nathalie Tesson, Vincent Vabret, Astrid Real-Time RT-PCR Detection of Equine Coronavirus |
title | Real-Time RT-PCR Detection of Equine Coronavirus |
title_full | Real-Time RT-PCR Detection of Equine Coronavirus |
title_fullStr | Real-Time RT-PCR Detection of Equine Coronavirus |
title_full_unstemmed | Real-Time RT-PCR Detection of Equine Coronavirus |
title_short | Real-Time RT-PCR Detection of Equine Coronavirus |
title_sort | real-time rt-pcr detection of equine coronavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120059/ http://dx.doi.org/10.1007/978-1-4939-3414-0_8 |
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