Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome

We have developed a DNA assembly platform that utilizes the nonspecific, highly variable sequence signatures of type IIs restriction enzymes to assemble a full-length molecular clone of murine hepatitis coronavirus (MHV) strain A59. The approach also allows changes to be engineered into a DNA fragme...

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Detalles Bibliográficos
Autores principales: Donaldson, Eric F., Sims, Amy C., Baric, Ralph S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120124/
https://www.ncbi.nlm.nih.gov/pubmed/19057869
http://dx.doi.org/10.1007/978-1-59745-181-9_21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120124/
https://www.ncbi.nlm.nih.gov/pubmed/19057869
http://dx.doi.org/10.1007/978-1-59745-181-9_21

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