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Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome

We have developed a DNA assembly platform that utilizes the nonspecific, highly variable sequence signatures of type IIs restriction enzymes to assemble a full-length molecular clone of murine hepatitis coronavirus (MHV) strain A59. The approach also allows changes to be engineered into a DNA fragme...

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Detalles Bibliográficos
Autores principales:	Donaldson, Eric F., Sims, Amy C., Baric, Ralph S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2007
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120124/ https://www.ncbi.nlm.nih.gov/pubmed/19057869 http://dx.doi.org/10.1007/978-1-59745-181-9_21

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