Virus Neutralization Assay for Turkey Coronavirus Infection

Turkey coronavirus (TCoV) infection induces the production of protective antibodies against the sequent exposure of TCoV. Serological tests to determine TCoV-specific antibodies are critical to evaluate previous exposure to TCoV in the turkey flocks and differentiate serotypes from different isolate...

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Autores principales: Chen, Yi-Ning, Wu, Ching Ching, Lin, Tsang Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120153/
http://dx.doi.org/10.1007/978-1-4939-3414-0_3
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author Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
author_facet Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
author_sort Chen, Yi-Ning
collection PubMed
description Turkey coronavirus (TCoV) infection induces the production of protective antibodies against the sequent exposure of TCoV. Serological tests to determine TCoV-specific antibodies are critical to evaluate previous exposure to TCoV in the turkey flocks and differentiate serotypes from different isolates or strains. A specific virus neutralization assay using embryonated turkey eggs and immunofluorescent antibody assay for determining TCoV-specific neutralizing antibodies is described in this chapter. Virus neutralization titer of turkey serum from turkeys infected with TCoV is the dilution of serum that can inhibit TCoV infection in 50 % of embryonated turkey eggs. Virus neutralization assay for TCoV is useful to monitor the immune status of turkey flocks infected with TCoV for the control of the disease.
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spelling pubmed-71201532020-04-06 Virus Neutralization Assay for Turkey Coronavirus Infection Chen, Yi-Ning Wu, Ching Ching Lin, Tsang Long Animal Coronaviruses Article Turkey coronavirus (TCoV) infection induces the production of protective antibodies against the sequent exposure of TCoV. Serological tests to determine TCoV-specific antibodies are critical to evaluate previous exposure to TCoV in the turkey flocks and differentiate serotypes from different isolates or strains. A specific virus neutralization assay using embryonated turkey eggs and immunofluorescent antibody assay for determining TCoV-specific neutralizing antibodies is described in this chapter. Virus neutralization titer of turkey serum from turkeys infected with TCoV is the dilution of serum that can inhibit TCoV infection in 50 % of embryonated turkey eggs. Virus neutralization assay for TCoV is useful to monitor the immune status of turkey flocks infected with TCoV for the control of the disease. 2015-09-10 /pmc/articles/PMC7120153/ http://dx.doi.org/10.1007/978-1-4939-3414-0_3 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
Virus Neutralization Assay for Turkey Coronavirus Infection
title Virus Neutralization Assay for Turkey Coronavirus Infection
title_full Virus Neutralization Assay for Turkey Coronavirus Infection
title_fullStr Virus Neutralization Assay for Turkey Coronavirus Infection
title_full_unstemmed Virus Neutralization Assay for Turkey Coronavirus Infection
title_short Virus Neutralization Assay for Turkey Coronavirus Infection
title_sort virus neutralization assay for turkey coronavirus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120153/
http://dx.doi.org/10.1007/978-1-4939-3414-0_3
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