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Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones

Some DNA fragments are difficult to clone in Escherichia coli by standard methods. It has been speculated that unintended transcription and translation result in expression of proteins that are toxic to the bacteria. This problem is frequently observed during assembly of infectious full-length virus...

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Detalles Bibliográficos
Autores principales:	Johansen, I. Elisabeth, Lund, Ole Søgaard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2008
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120191/ https://www.ncbi.nlm.nih.gov/pubmed/18370279 http://dx.doi.org/10.1007/978-1-59745-102-4_36

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