Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions

Severe acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-C...

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Autores principales: Peiris, J.S. Malik, Poon, Leo L.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120234/
https://www.ncbi.nlm.nih.gov/pubmed/19057863
http://dx.doi.org/10.1007/978-1-59745-181-9_6
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author Peiris, J.S. Malik
Poon, Leo L.M.
author_facet Peiris, J.S. Malik
Poon, Leo L.M.
author_sort Peiris, J.S. Malik
collection PubMed
description Severe acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-CoV RNA detection. In this chapter we describe a two-step conventional RT-PCR and a one-step quantitative RT-PCR that were used routinely in our laboratories during the SARS outbreak.
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spelling pubmed-71202342020-04-06 Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions Peiris, J.S. Malik Poon, Leo L.M. SARS- and Other Coronaviruses Article Severe acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-CoV RNA detection. In this chapter we describe a two-step conventional RT-PCR and a one-step quantitative RT-PCR that were used routinely in our laboratories during the SARS outbreak. 2007-11-28 /pmc/articles/PMC7120234/ /pubmed/19057863 http://dx.doi.org/10.1007/978-1-59745-181-9_6 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Peiris, J.S. Malik
Poon, Leo L.M.
Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title_full Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title_fullStr Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title_full_unstemmed Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title_short Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
title_sort detection of sars coronavirus in humans and animals by conventional and quantitative (real time) reverse transcription polymerase chain reactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120234/
https://www.ncbi.nlm.nih.gov/pubmed/19057863
http://dx.doi.org/10.1007/978-1-59745-181-9_6
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