Next-Generation Sequencing for Porcine Coronaviruses

The outbreak of porcine epidemic diarrhea virus and the discovery of porcine deltacoronavirus in the USA have led to multiple questions about the evolution of coronaviruses in swine. Coronaviruses are enveloped virus, containing a positive-sense single-stranded RNA genome (26–30 kb) that can cause r...

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Autores principales: Marthaler, Douglas, Bohac, Ann, Becker, Aaron, Peterson, Nichole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120291/
http://dx.doi.org/10.1007/978-1-4939-3414-0_19
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author Marthaler, Douglas
Bohac, Ann
Becker, Aaron
Peterson, Nichole
author_facet Marthaler, Douglas
Bohac, Ann
Becker, Aaron
Peterson, Nichole
author_sort Marthaler, Douglas
collection PubMed
description The outbreak of porcine epidemic diarrhea virus and the discovery of porcine deltacoronavirus in the USA have led to multiple questions about the evolution of coronaviruses in swine. Coronaviruses are enveloped virus, containing a positive-sense single-stranded RNA genome (26–30 kb) that can cause respiratory or enteric illness in swine. With current technologies, the complete viral genomes can be determined to understand viral diversity and evolution. In this chapter, we describe a method to deep genome sequence porcine coronavirus on the Illumina MiSeq, avoiding the number of contaminating reads associated with the host and other microorganisms.
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spelling pubmed-71202912020-04-06 Next-Generation Sequencing for Porcine Coronaviruses Marthaler, Douglas Bohac, Ann Becker, Aaron Peterson, Nichole Animal Coronaviruses Article The outbreak of porcine epidemic diarrhea virus and the discovery of porcine deltacoronavirus in the USA have led to multiple questions about the evolution of coronaviruses in swine. Coronaviruses are enveloped virus, containing a positive-sense single-stranded RNA genome (26–30 kb) that can cause respiratory or enteric illness in swine. With current technologies, the complete viral genomes can be determined to understand viral diversity and evolution. In this chapter, we describe a method to deep genome sequence porcine coronavirus on the Illumina MiSeq, avoiding the number of contaminating reads associated with the host and other microorganisms. 2015-09-10 /pmc/articles/PMC7120291/ http://dx.doi.org/10.1007/978-1-4939-3414-0_19 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Marthaler, Douglas
Bohac, Ann
Becker, Aaron
Peterson, Nichole
Next-Generation Sequencing for Porcine Coronaviruses
title Next-Generation Sequencing for Porcine Coronaviruses
title_full Next-Generation Sequencing for Porcine Coronaviruses
title_fullStr Next-Generation Sequencing for Porcine Coronaviruses
title_full_unstemmed Next-Generation Sequencing for Porcine Coronaviruses
title_short Next-Generation Sequencing for Porcine Coronaviruses
title_sort next-generation sequencing for porcine coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120291/
http://dx.doi.org/10.1007/978-1-4939-3414-0_19
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