Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System

The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to...

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Autores principales: Khan, Sehaam, Ng, Mah-Lee, Tan, Yee-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120620/
https://www.ncbi.nlm.nih.gov/pubmed/17502669
http://dx.doi.org/10.1007/978-1-59745-393-6_3
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author Khan, Sehaam
Ng, Mah-Lee
Tan, Yee-Joo
author_facet Khan, Sehaam
Ng, Mah-Lee
Tan, Yee-Joo
author_sort Khan, Sehaam
collection PubMed
description The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy.
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spelling pubmed-71206202020-04-06 Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System Khan, Sehaam Ng, Mah-Lee Tan, Yee-Joo Glycovirology Protocols Article The Bac-to-Bac Baculovirus expression system was used to generate a recombinant baculovirus capable of expressing the severe acute respiratory syndrome (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy. 2007 /pmc/articles/PMC7120620/ /pubmed/17502669 http://dx.doi.org/10.1007/978-1-59745-393-6_3 Text en © Humana Press Inc., Totowa, NJ 2007 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Khan, Sehaam
Ng, Mah-Lee
Tan, Yee-Joo
Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title_full Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title_fullStr Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title_full_unstemmed Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title_short Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System
title_sort expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the baculovirus expression system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120620/
https://www.ncbi.nlm.nih.gov/pubmed/17502669
http://dx.doi.org/10.1007/978-1-59745-393-6_3
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