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Screening and Identification of Virus-Encoded RNA Silencing Suppressors
RNA silencing, including RNA interference, is a novel method of gene regulation and one of the potent host-defense mechanisms against the viruses. In the course of evolution, the viruses have encoded proteins with the potential to suppress the host RNA silencing mechanism as a counterdefense strateg...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120842/ https://www.ncbi.nlm.nih.gov/pubmed/18369787 http://dx.doi.org/10.1007/978-1-59745-191-8_14 |
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author | Karjee, Sumona Islam, Mohammad Nurul Mukherjee, Sunil K. |
author_facet | Karjee, Sumona Islam, Mohammad Nurul Mukherjee, Sunil K. |
author_sort | Karjee, Sumona |
collection | PubMed |
description | RNA silencing, including RNA interference, is a novel method of gene regulation and one of the potent host-defense mechanisms against the viruses. In the course of evolution, the viruses have encoded proteins with the potential to suppress the host RNA silencing mechanism as a counterdefense strategy. The virus-encoded RNA silencing suppressors (RSSs) can serve as important biological tools to dissect the detailed RNA silencing pathways and also to evolve the antiviral strategies. Screening and identification of the RSSs are indeed of utmost significance in the field of plant biotechnology. We describe two Green Fluorescent Protein (GFP) reporter-based plant assay systems that rely on two different principles, namely reversal of silencing and enhancement of rolling circle replication (RCR) of geminiviral replicon. These proof-of-concept examples and assay systems could be used to screen various plant, animal, and insect viral ORFs for identification of the RSS activities. |
format | Online Article Text |
id | pubmed-7120842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71208422020-04-06 Screening and Identification of Virus-Encoded RNA Silencing Suppressors Karjee, Sumona Islam, Mohammad Nurul Mukherjee, Sunil K. RNAi Article RNA silencing, including RNA interference, is a novel method of gene regulation and one of the potent host-defense mechanisms against the viruses. In the course of evolution, the viruses have encoded proteins with the potential to suppress the host RNA silencing mechanism as a counterdefense strategy. The virus-encoded RNA silencing suppressors (RSSs) can serve as important biological tools to dissect the detailed RNA silencing pathways and also to evolve the antiviral strategies. Screening and identification of the RSSs are indeed of utmost significance in the field of plant biotechnology. We describe two Green Fluorescent Protein (GFP) reporter-based plant assay systems that rely on two different principles, namely reversal of silencing and enhancement of rolling circle replication (RCR) of geminiviral replicon. These proof-of-concept examples and assay systems could be used to screen various plant, animal, and insect viral ORFs for identification of the RSS activities. 2008 /pmc/articles/PMC7120842/ /pubmed/18369787 http://dx.doi.org/10.1007/978-1-59745-191-8_14 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Karjee, Sumona Islam, Mohammad Nurul Mukherjee, Sunil K. Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title | Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title_full | Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title_fullStr | Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title_full_unstemmed | Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title_short | Screening and Identification of Virus-Encoded RNA Silencing Suppressors |
title_sort | screening and identification of virus-encoded rna silencing suppressors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120842/ https://www.ncbi.nlm.nih.gov/pubmed/18369787 http://dx.doi.org/10.1007/978-1-59745-191-8_14 |
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